Rat CD284/TLR4 protein (Recombinant) (N-His) (STJP014915)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenRattus norvegicus (Rat)
General Information
| Short Description | Recombinant-Rat CD284/TLR4-N-His protein was developed from e.coli and has a target region of N-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 5% Trehalose. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | Please contact us for this information. |
Target Information
| Gene Symbol | Tlr4 |
| Gene ID | 29260 |
| Uniprot ID | TLR4_RAT |
| Immunogen | Rattus norvegicus (Rat) |
| Immunogen Region | His49-Gln247 |
Additional Info
| Function | Transmembrane receptor that functions as a pattern recognition receptor recognizing pathogen- and damage-associated molecular patterns (PAMPs and DAMPs) to induce innate immune responses via downstream signaling pathways. At the plasma membrane, cooperates with LY96 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+). Mechanistically, acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Alternatively, CD14-mediated TLR4 internalization via endocytosis is associated with the initiation of a MYD88-independent signaling via the TICAM1-TBK1-IRF3 axis leading to type I interferon production. In addition to the secretion of proinflammatory cytokines, initiates the activation of NLRP3 inflammasome and formation of a positive feedback loop between autophagy and NF-kappa-B signaling cascade. In complex with TLR6, promotes inflammation in monocytes/macrophages by associating with TLR6 and the receptor CD86. Upon ligand binding, such as oxLDL or amyloid-beta 42, the TLR4:TLR6 complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway. In myeloid dendritic cells, vesicular stomatitis virus glycoprotein G but not LPS promotes the activation of IRF7, leading to type I IFN production in a CD14-dependent manner. |
| Protein Name | Toll-Like Receptor 4Toll4Cd Antigen Cd284 |
| Database Links | Reactome: R-RNO-140534Reactome: -RNO-166016Reactome: -RNO-166166Reactome: -RNO-2562578Reactome: -RNO-5686938Reactome: -RNO-936964Reactome: -RNO-937041Reactome: -RNO-937072Reactome: -RNO-9707616Reactome: -RNO-975163Reactome: -RNO-9824878 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinEarly EndosomeCell ProjectionRuffleUpon Complex Formation With Cd36 And Tlr6Internalized Through Dynamin-Dependent EndocytosisColocalizes With Rftn1 At Cell Membrane And Then Together With Rftn1 Moves To EndosomesUpon Lipopolysaccharide Stimulation |
| Alternative Protein Names | Toll-Like Receptor 4 proteinToll4 proteinCd Antigen Cd284 proteinTlr4 protein |
Information sourced from Uniprot.org