Rat CCL2/MCP-1 protein (Recombinant-Active) (C-His) (STJP011875)

SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenRattus norvegicus (Rat)
STJP011875-100
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General Information

Short DescriptionRecombinant-Active-Rat CCL2/MCP-1-C-His protein was developed from mammalian cells and has a target region of C-His. For use in research applications.
ApplicationsELISA/WB
HostMammalian Cells
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ConjugationUnconjugated
Dilution RangeReconstitute in sterile water for a stock solution.
FormulationLyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol.
Storage InstructionUse a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt.
Endotoxin<0.1 EU/Mu g of the protein by the LAL method.
ImmunoreactivityMeasured by its ability to chemoattract mouse pro‑B cells (BaF3) transfected with human CCR2A. The ED50 for this effect is 30-150 ng/mL.

Target Information

Gene SymbolCcl2
Gene ID24770
Uniprot IDCCL2_RAT
ImmunogenRattus norvegicus (Rat)
Immunogen RegionGln24-Asn148

Additional Info

Function Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B.
Protein Name C-C Motif Chemokine 2
Immediate-Early Serum-Responsive Protein Je
Monocyte Chemoattractant Protein 1
Monocyte Chemotactic Protein 1
Mcp-1
Small-Inducible Cytokine A2
Database Links
Cellular Localisation Secreted
Alternative Protein Names C-C Motif Chemokine 2 protein
Immediate-Early Serum-Responsive Protein Je protein
Monocyte Chemoattractant Protein 1 protein
Monocyte Chemotactic Protein 1 protein
Mcp-1 protein
Small-Inducible Cytokine A2 protein
Ccl2 protein
Je protein
Mcp1 protein
Scya2 protein

Information sourced from Uniprot.org

Citations

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