| Protein Name | Complement C4 Cleaved Into - Complement C4 Beta Chain - Complement C4 Alpha Chain - C4a Anaphylatoxin - Complement C4 Gamma Chain |
| Database Links | Reactome: R-RNO-166663Reactome: -RNO-174577Reactome: -RNO-381426Reactome: -RNO-8957275Reactome: -RNO-977606 |
| Cellular Localisation | SecretedSynapseCell ProjectionAxonDendrite |
| Alternative ELISA Names | Complement C4 Cleaved Into - Complement C4 Beta Chain - Complement C4 Alpha Chain - C4a Anaphylatoxin - Complement C4 Gamma Chain ELISA kitC4 ELISA kitC4a ELISA kit |
| Specificity | This kit recognizes Rat C4 in samples. No significant cross-reactivity or interference between Rat C4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-109 | 100 |
| EDTA plasma (n=8) | 94-107 | 101 |
| Cell culture media (n=8) | 86-99 | 91 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 10.49 | 19.29 | 75.42 | 10.97 | 17.67 | 77.38 |
| Standard deviation | 0.58 | 0.92 | 3.93 | 0.65 | 0.84 | 3.75 |
| CV (%) | 5.53 | 4.77 | 5.21 | 5.93 | 4.75 | 4.85 |
