| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
7.5pg/mL |
| Detection Limit: |
12.5~800pg/mL |
| Short Description: |
This rat BAD kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Bad |
| Gene ID: |
64639 |
| Uniprot ID: |
BAD_RAT |
| Specificity: |
This kit recognizes Rat BAD in samples. No significant cross-reactivity or interference between Rat BAD and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in all tissues tested, including brain, liver, spleen and heart. In the brain, restricted to epithelial cells of the choroid plexus. Isoform alpha is the more abundant form. |
| Post Translational Modifications | Phosphorylated at one or more of Ser-113, Ser-137, Ser-156 and Ser-171 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-137 or Ser-113 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-156, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-137 is the major site of AKT/PKB phosphorylation, Ser-156 the major site of protein kinase A (CAPK) phosphorylation. Methylation at Arg-132 and Arg-134 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-137. |
| Function | Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. |
| Protein Name | Bcl2-Associated Agonist Of Cell DeathBadBcl-2-Binding Component 6Bcl-Xl/Bcl-2-Associated Death PromoterBcl2 Antagonist Of Cell Death |
| Database Links | Reactome: R-RNO-111447Reactome: -RNO-111453Reactome: -RNO-193648 |
| Cellular Localisation | Mitochondrion Outer MembraneCytoplasmColocalizes With Hif3a In The CytoplasmUpon PhosphorylationLocates To The Cytoplasm |
| Alternative CLIA Names | Bcl2-Associated Agonist Of Cell Death CLIA kitBad CLIA kitBcl-2-Binding Component 6 CLIA kitBcl-Xl/Bcl-2-Associated Death Promoter CLIA kitBcl2 Antagonist Of Cell Death CLIA kitBad CLIA kit |
| Specificity | This kit recognizes Rat BAD in samples. No significant cross-reactivity or interference between Rat BAD and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 99-113 | 105 |
| EDTA plasma(n=8) | 100-114 | 108 |
| Cell culture media(n=8) | 95-109 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 39.51 | 68.42 | 399.64 | 38.60 | 65.23 | 435.19 |
| Standard deviation | 4.34 | 7.61 | 39.72 | 3.84 | 6.64 | 48.61 |
| CV (%) | 10.98 | 11.12 | 9.94 | 9.95 | 10.18 | 11.17 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
