This rat BAD kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications
CLIA
Reactivity
Rat
Sensitivity
7.5pg/mL
Detection Limit
12.5~800pg/mL
Note
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Product Properties
Storage Instruction
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
This kit recognizes Rat BAD in samples. No significant cross-reactivity or interference between Rat BAD and analogues was observed.
Sample Type
Serum, plasma and other biological fluids
Additional Info
Tissue Specificity
Expressed in all tissues tested, including brain, liver, spleen and heart. In the brain, restricted to epithelial cells of the choroid plexus. Isoform alpha is the more abundant form.
Post Translational Modifications
Phosphorylated at one or more of Ser-113, Ser-137, Ser-156 and Ser-171 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-137 or Ser-113 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-156, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-137 is the major site of AKT/PKB phosphorylation, Ser-156 the major site of protein kinase A (CAPK) phosphorylation. Methylation at Arg-132 and Arg-134 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-137.
Function
Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Protein Name
Bcl2-Associated Agonist Of Cell DeathBadBcl-2-Binding Component 6Bcl-Xl/Bcl-2-Associated Death PromoterBcl2 Antagonist Of Cell Death
Mitochondrion Outer MembraneCytoplasmColocalizes With Hif3a In The CytoplasmUpon PhosphorylationLocates To The Cytoplasm
Alternative CLIA Names
Bcl2-Associated Agonist Of Cell Death CLIA kitBad CLIA kitBcl-2-Binding Component 6 CLIA kitBcl-Xl/Bcl-2-Associated Death Promoter CLIA kitBcl2 Antagonist Of Cell Death CLIA kitBad CLIA kit
Specificity
This kit recognizes Rat BAD in samples. No significant cross-reactivity or interference between Rat BAD and analogues was observed.
