| Applications: | CLIA |
| Reactivity: | Rat |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 3.75ng/mL |
| Detection Limit: | 6.25~400ng/mL |
| Short Description : | This rat ApoA1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
| Gene Symbol: | Apoa1 |
| Gene ID: | 25081 |
| Uniprot ID: | APOA1_RAT |
| Specificity: | This kit recognizes Rat ApoA1 in samples. No significant cross-reactivity or interference between Rat ApoA1 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Tissue Specificity | Major protein of plasma HDL, also found in chylomicrons. |
| Post Translational Modifications | Glycosylated. Palmitoylated. Phosphorylation sites are present in the extracellular medium. |
| Function | Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. |
| Protein Name | Apolipoprotein A-IApo-AiApoa-IApolipoprotein A1 Cleaved Into - Proapolipoprotein A-IProapoa-I |
| Database Links | Reactome: R-RNO-114608Reactome: -RNO-1369062Reactome: -RNO-2168880Reactome: -RNO-3000471Reactome: -RNO-3000480Reactome: -RNO-381426Reactome: -RNO-8957275Reactome: -RNO-8963888Reactome: -RNO-8963896Reactome: -RNO-8963901Reactome: -RNO-8964011Reactome: -RNO-8964058Reactome: -RNO-9707616Reactome: -RNO-975634 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Apolipoprotein A-I CLIA kitApo-Ai CLIA kitApoa-I CLIA kitApolipoprotein A1 Cleaved Into - Proapolipoprotein A-I CLIA kitProapoa-I CLIA kitApoa1 CLIA kit |
| Specificity | This kit recognizes Rat ApoA1 in samples. No significant cross-reactivity or interference between Rat ApoA1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 84-98 | 91 |
| EDTA plasma(n=8) | 94-107 | 99 |
| Cell culture media(n=8) | 95-108 | 100 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 21.68 | 53.68 | 181.77 | 22.00 | 57.04 | 196.28 |
| Standard deviation | 1.78 | 5.00 | 12.36 | 2.57 | 5.40 | 16.98 |
| CV (%) | 8.21 | 9.31 | 6.80 | 11.68 | 9.47 | 8.65 |
