| Host: | E.coli |
| Applications: | Enzyme Activity/SDS-PAGE |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-rat ALT1/GPT1-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Formulation: | Liquid in phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol |
| Concentration: | 1 mg/mL |
| Storage Instruction: | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: | Specific activity is > 60unit/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C. |
| Gene Symbol: | Gpt |
| Gene ID: | 81670 |
| Uniprot ID: | ALAT1_RAT |
| Immunogen Region: | 1-496aa |
| Accession Number: | NP_112301 |
| Immunogen: | Rat |
| Immunogen Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMASRVND QSQASRNGLK GKVLTLDTMN PCVRRVEYAV RGPIVQRALE LEQELRQGVK KPFTEVIRAN IGDAQAMGQR PITFFRQVLA LCVYPNLLSS PDFPEDAKRR AERILQACGG HSLGAYSISS GIQPIREDVA QYIERRDGGI PADPNNIFLS TGASDAIVTM LKLLVSGEGR ARTGVLIPIP QYPLYSAALA ELDAVQVD |
| Tissue Specificity | Liver, heart, skeletal muscle, etc. |
| Function | Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. |
| Protein Name | Alanine Aminotransferase 1Alt1Glutamate Pyruvate Transaminase 1Gpt 1Glutamic--Alanine Transaminase 1Glutamic--Pyruvic Transaminase 1 |
| Database Links | Reactome: R-RNO-70268Reactome: -RNO-8964540 |
| Cellular Localisation | Cytoplasm |
| Alternative Protein Names | Alanine Aminotransferase 1 proteinAlt1 proteinGlutamate Pyruvate Transaminase 1 proteinGpt 1 proteinGlutamic--Alanine Transaminase 1 proteinGlutamic--Pyruvic Transaminase 1 proteinGpt proteinAat1 proteinGpt1 protein |
Information sourced from Uniprot.org
