| Applications: | CLIA |
| Reactivity: | Rat |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 4.69ng/mL |
| Detection Limit: | 7.81~500ng/mL |
| Short Description : | This rat AGT kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
| Gene Symbol: | Agt |
| Gene ID: | 24179 |
| Uniprot ID: | ANGT_RAT |
| Specificity: | This kit recognizes Rat AGT in samples. No significant cross-reactivity or interference between Rat AGT and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | In response to low blood pressure, the enzyme renin/REN cleaves angiotensinogen to produce angiotensin-1. Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4. Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2 and can be further processed by ACE to produce angiotensin 1-7, angiotensin 1-5 and angiotensin 1-4. Angiotensin 1-7 has also been proposed to be cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). The disulfide bond is labile. Angiotensinogen is present in the circulation in a near 40:60 ratio with the oxidized disulfide-bonded form, which preferentially interacts with receptor-bound renin. |
| Function | Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. Angiotensin-2: Acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone. Acts by binding to angiotensin receptors AGTR1 and AGTR2. Also binds the DEAR/FBXW7-AS1 receptor. Angiotensin-3: Stimulates aldosterone release. Angiotensin 1-7: Is a ligand for the G-protein coupled receptor MAS1. Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets. |
| Protein Name | Angiotensinogen Serpin A8 Cleaved Into - Angiotensin-1 Angiotensin 1-10 Angiotensin I Ang I - Angiotensin-2 Angiotensin 1-8 Angiotensin Ii Ang Ii - Angiotensin-3 Angiotensin 2-8 Angiotensin Iii Ang Iii Des-Asp1-Angiotensin Ii - Angiotensin-4 Angiotensin 3-8 Angiotensin Iv Ang Iv - Angiotensin 1-9 - Angiotensin 1-7 - Angiotensin 1-5 - Angiotensin 1-4 |
| Database Links | Reactome: R-RNO-2022377 Reactome: -RNO-375276 Reactome: -RNO-416476 Reactome: -RNO-418594 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Angiotensinogen CLIA kit Serpin A8 Cleaved Into - Angiotensin-1 CLIA kit Angiotensin 1-10 CLIA kit Angiotensin I CLIA kit Ang I - Angiotensin-2 CLIA kit Angiotensin 1-8 CLIA kit Angiotensin Ii CLIA kit Ang Ii - Angiotensin-3 CLIA kit Angiotensin 2-8 CLIA kit Angiotensin Iii CLIA kit Ang Iii CLIA kit Des-Asp1-Angiotensin Ii - Angiotensin-4 CLIA kit Angiotensin 3-8 CLIA kit Angiotensin Iv CLIA kit Ang Iv - Angiotensin 1-9 - Angiotensin 1-7 - Angiotensin 1-5 - Angiotensin 1-4 CLIA kit Agt CLIA kit Serpina8 CLIA kit |
| Specificity | This kit recognizes Rat AGT in samples. No significant cross-reactivity or interference between Rat AGT and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 98-111 | 105 |
| EDTA plasma(n=8) | 89-104 | 96 |
| Cell culture media(n=8) | 97-111 | 103 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 26.93 | 78.00 | 189.83 | 25.89 | 75.88 | 189.94 |
| Standard deviation | 3.39 | 7.53 | 21.49 | 2.75 | 8.97 | 16.22 |
| CV (%) | 12.59 | 9.65 | 11.32 | 10.62 | 11.82 | 8.54 |