| Applications: | ELISA |
| Reactivity: | Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.64ng/mL |
| Detection Limit: | 1.56-100ng/mL |
| Short Description : | This ACTb Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Storage Instruction: | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 3 hrs |
| Gene Symbol: | Actb |
| Gene ID: | 81822 |
| Uniprot ID: | ACTB_RAT |
| Immunogen Region: | Ready-To-Use |
| Sample Type: | tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Tissue Specificity | Expressed in the epididymis (at protein level). |
| Post Translational Modifications | Actin, cytoplasmic 1: N-terminal cleavage of acetylated methionine of immature cytoplasmic actin by ACTMAP. ISGylated. Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization. Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. Methylated at His-73 by SETD3. Methylation at His-73 is required for smooth muscle contraction of the laboring uterus during delivery. Actin, cytoplasmic 1, N-terminally processed: N-terminal acetylation by NAA80 affects actin filament depolymerization and elongation, including elongation driven by formins. In contrast, filament nucleation by the Arp2/3 complex is not affected. |
| Function | Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA. Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules. |
| Protein Name | Actin - Cytoplasmic 1Beta-Actin Cleaved Into - Actin - Cytoplasmic 1 - N-Terminally Processed |
| Database Links | Reactome: R-RNO-190873Reactome: -RNO-196025Reactome: -RNO-2029482Reactome: -RNO-3928662Reactome: -RNO-418990Reactome: -RNO-437239Reactome: -RNO-4420097Reactome: -RNO-445095Reactome: -RNO-446353Reactome: -RNO-5250924Reactome: -RNO-5626467Reactome: -RNO-5663213Reactome: -RNO-5663220Reactome: -RNO-5674135Reactome: -RNO-5689603Reactome: -RNO-5696394Reactome: -RNO-8856828Reactome: -RNO-9035034 |
| Cellular Localisation | CytoplasmCytoskeletonNucleusLocalized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas |
| Alternative ELISA Names | Actin - Cytoplasmic 1 ELISA kitBeta-Actin Cleaved Into - Actin - Cytoplasmic 1 - N-Terminally Processed ELISA kitActb ELISA kit |
| output |
Information sourced from Uniprot.org
