| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Phospho-PRP19 Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Dilution Range: |
WB: 1:500-1:1, 000 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Function | Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome. Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex. Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries. The XAB2 complex, which contains PRPF19, is also involved in pre-mRNA splicing, transcription and transcription-coupled repair. Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. 'Lys-63'-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response. May also play a role in DNA double-strand break (DSB) repair by recruiting the repair factor SETMAR to altered DNA. As part of the PSO4 complex may also be involved in the DNA interstrand cross-links/ICLs repair process. In addition, may also mediate 'Lys-48'-linked polyubiquitination of substrates and play a role in proteasomal degradation. May play a role in the biogenesis of lipid droplets. May play a role in neural differentiation possibly through its function as part of the spliceosome. |
| Peptide Name | Pre-Mrna-Processing Factor 19Neuronal Differentiation-Related Gene ProteinPrp19/Pso4 HomologRing-Type E3 Ubiquitin Transferase Prp19 |
| Database Links | Reactome: R-RNO-6781823Reactome: -RNO-6782135Reactome: -RNO-6782210Reactome: -RNO-72163 |
| Cellular Localisation | NucleusNucleoplasmCytoplasmCytoskeletonSpindleLipid DropletNucleoplasmic In Interphase CellsIrregularly Distributed In Anaphase CellsIn Prophase CellsUniformly DistributedBut Not Associated With Condensing ChromosomesFound In Extrachromosomal Regions In Metaphase CellsMainly Localized To The Mitotic Spindle Apparatus When Chromosomes Segregate During AnaphaseWhen Nuclei Reform During Late TelophaseUniformly Distributed In Daughter Cells And Displays No Preferred Association With Decondensing ChromatinRecruited On Damaged Dna At Sites Of Double-Strand Break |
| Alternative Peptide Names | Pre-Mrna-Processing Factor 19 proteinNeuronal Differentiation-Related Gene Protein proteinPrp19/Pso4 Homolog proteinRing-Type E3 Ubiquitin Transferase Prp19 proteinPrpf19 proteinPrp19 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
