Methyl Antibodies | St John's Laboratory

Immunofluorescence analysis of HeLa cells using MonoMethyl-NONO/p54nrb-R357/R365 pAb (STJ110387) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Mono-Methyl-NONO-R357/R365 antibody (STJ110387)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

£126.50 - £618.50

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Western blot analysis of MCF7 cells using Mono-Methyl-NF Kappa B-p65 (K314/K315) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Mono-Methyl-RELA-Lys314/Lys315 antibody (STJ90114)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 293T COLO using TP53 antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-Mono-Methyl-TP53-Lys370 antibody (STJ97694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma using P53 (Mono Methyl Lys370) Mouse mAb diluted at 1:500.

Anti-Mono-Methyl-TP53-Q59 antibody [Q59] (STJ97495)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-UHRF1-K385 antibody (STJ118448) at 1:1000 dilution.

Anti-Mono-Methyl-UHRF1-K385 antibody (STJ118448)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates using MonoMethyl-YAP1-K342 Rabbit polyclonal antibody (STJ11100581) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-Mono-Methyl-YAP1-K342 antibody (STJ11100581)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunohistochemistry analysis of MonoMethyl-Histone H2B-K116 in paraffin-embedded rat heart using MonoMethyl-Histone H2B-K116 Rabbit monoclonal antibody (STJ11103791) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-MonoMethyl-Histone H2B-K116 antibody [S3791RM] (STJ11103791)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£143.50 - £618.50

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Anti-MonoMethyl-Histone H3-K27 antibody [SRM14499] (STJ11109229)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/A.thaliana/Rice/Other

£0.00

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Anti-MonoMethyl-Histone H3-K36 antibody [SRM13261] (STJ11107991)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£0.00

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Anti-MonoMethyl-Histone H3-K36 antibody [SRM13352] (STJ11108082)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/IP/ELISA/ChIP

Reactivity :

Human/Mouse/Rat/Other

£0.00

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Anti-MonoMethyl-Histone H3-K4 antibody [SRM15050] (STJ11109780)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IHC-P/DB/ChIP/ELISA

Reactivity :

Human/Mouse/Rat/A.thaliana/Rice/Wheat/Other

£0.00

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Anti-MonoMethyl-Histone H3-K79 antibody [SRM13169] (STJ11107899)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£0.00

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Anti-MonoMethyl-Histone H3-K79 antibody [SRM13213] (STJ11107943)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£0.00

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Anti-MonoMethyl-Histone H3-K9 antibody [SRM13076] (STJ11107806)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA/ChIP/CUT&Tag

Reactivity :

Human/Mouse/Rat/Other

£0.00

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Anti-MonoMethyl-Histone H3-K9 antibody [SRM14481] (STJ11109211)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£0.00

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Anti-MonoMethyl-Histone H3-Lys36 antibody [PT0927R] (STJA0039922)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-MonoMethyl-Histone H3-Lys9 antibody [PT0921R] (STJA0039818)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA/CHIP/Cut&Tag

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-MonoMethyl-Histone H4-K20 antibody [SRM13245] (STJ11107975)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£0.00

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Anti-MonoMethyl-Histone H4-Lys20 antibody [PT1059R] (STJA0039971)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Immunofluorescence analysis of U-2 OS cells using MonoMethyl-Histone H4-R3 Rabbit pAb (STJ11106422) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MonoMethyl-Histone H4-R3 antibody (STJ11106422)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Immunofluorescence analysis of HeLa cells using N5-Methylcytidine Rabbit polyclonal antibody (STJ29880) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-N5-Methyl-m5C antibody (STJ29880)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£110.50 - £618.50

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The m6A mouse monoclonal antibody (STJ11100594) are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine (100ng for each oligomer). Oligomer 8- N6-methyladenosine (ATAACTGG-m6A-CCGAATGG) Oligomer 9- unmodified adenosine (ATAACTGGACCGAATGG) Oligomer 10- unmodified adenosine (AAAAAAAAAAAAAAAA-biotin)

Anti-N6-Methyl-m6A antibody [S4MM] (STJ11100594)

Clonality :

Monoclonal

Host :

Mouse

Applications :

DB/ELISA

Reactivity :

Species Independent

£118.50 - £521.50

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RNA Immunoprecipitation was performed on 100 µg mouse liver total RNA, using 5 µg of the N6-methyladenosine/ m6A Rabbit mAb. An equal amount of IgG was used as negative control. The immunoprecipitated RNA was verified by using HS6ST1 as PCR primer of qRT-PCR. The picture shows the relative enrichment multiple of HS6ST1 site.

Anti-N6-Methyl-m6A antibody [S4MR] (STJ11101784)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

DB/IF/ICC/ELISA/meRIP

Reactivity :

Species Independent

£143.50 - £618.50

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Western blot analysis of various lysates using Pan DiMethyl-lysine Rabbit polyclonal antibody (STJ11100252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-Pan-Di-Methyl-Kme2 antibody (STJ11100252)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of NIH/3T3 cells using Pan DiMethyl-lysine Rabbit polyclonal antibody (STJ27698) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Pan-Di-Methyl-Lysine antibody (STJ27698)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Dot-blot analysis of all sorts of methylation peptides using pan Symmetric-DiMethyl-Arginine antibody (STJ11100217) at 1:1000 dilution.

Anti-Pan-Di-Methyl-Rme2s antibody (STJ11100217)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Dot-blot analysis of all sorts using pan-methyl-Histidine polyclonal antibody (STJ11103884) at 1:1000 dilution. Peptide 1：Peptide library (3-methylhistidine-intermediate) Peptide 2：Peptide library (3-methylhistidine-C-terminal) Peptide 3：Peptide library (1-methylhistidine-intermediate) Peptide 4：Peptide library (1-methylhistidine-C-terminal) Peptide 5：Histidine methylation modified peptide library mixPeptide 6：Control peptide library mix

Anti-pan-Methyl-Histidine antibody (STJ11103884)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain tissue, diluted at 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-Pan-Methyl-Lysine antibody (STJ197343)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Species independent

£49.00 - £261.00

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Western blot analysis of Hela using Pan Methylated Lysine monoclonal antibody.

Anti-Pan-Methyl-Lysine-Mix antibody [Mix] (STJ97446)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Species independent

£49.00 - £261.00

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Western blot analysis of various lysates using pan-Mono-Methyl R Motif Rabbit polyclonal antibody (STJ11103886) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-pan-Mono-Methyl-Arginine Motif antibody (STJ11103886)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of extracts of various cell lines, using Pan Mono-Methyl lysine antibody (STJ11100249) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Pan-Mono-Methyl-Kme1 antibody (STJ11100249)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of extracts of various cell lines, using pan-Mono-MethylR (R

Anti-Pan-Mono-Methyl-Rme1 antibody (STJ11100253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates, using Pan-SymmetricDi-MethylArginineMotif Rabbit monoclonal antibody (STJ11103452) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Pan-Symmetric Di-Methyl-Arginine Motif antibody [S2MR] (STJ11103452)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using Pan-Symmetric Di-Methyl Arginine Motif antibody (STJ11104843) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-Pan-Symmetric Di-Methyl-Arginine Motif antibody [S4843RM] (STJ11104843)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£143.50 - £618.50

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Western blot analysis of various lysates using pan-TriMethyl-lysine polyclonal antibody (STJ11100248) at 1:500 dilution. HeLa and MCF7 cells were treated by ADOX (100 Mu M) for 24 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-Pan-Tri-Methyl-Kme3 antibody (STJ11100248)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Chromatin immunoprecipitation analysis of extracts of HeLa cells, using pan-Tri-Methyl lysine antibody (STJ11103178) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Pan-Tri-Methyl-Lysine antibody (STJ11103178)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA/ChIP

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Anti-Recombinant-Di-methyl-Histone H3-K4 antibody [RM1A58] (STJA0026729)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/IF/IHC/ChIP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-Di-methyl-Histone H3-K4 antibody [RM2B03] (STJA0025076)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/ChIP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-Di-methyl-Histone H3-K79 antibody [RM2A96] (STJA0025070)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/ChIP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-Di-methyl-Histone H3-K9 antibody [RM2A90] (STJA0025064)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ChIP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-Di-methyl-Histone H3-R17 antibody [RM2A95] (STJA0025069)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/WB

Reactivity :

Human/Mouse

£300.50 - £457.50

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Anti-Recombinant-Mono-methyl-Histone H2A-K118 antibody [RM1J80] (STJA0023470)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-Mono-methyl-Histone H2B-K116 antibody [RM2H54] (STJA0025721)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-Mono-methyl-Histone H2B-R79 antibody [RM1C12] (STJA0026881)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£300.50 - £457.50

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Anti-Recombinant-Mono-methyl-Histone H3-K14 antibody [RM2B02] (STJA0025075)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/IF/WB

Reactivity :

Human/Mouse

£300.50 - £457.50

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Anti-Recombinant-Mono-methyl-Histone H3-K18 antibody [RM1C11] (STJA0026880)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-Mono-methyl-Histone H3-K36 antibody [RM1C04] (STJA0026873)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/WB

Reactivity :

Human/Mouse

£300.50 - £457.50

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Anti-Recombinant-Mono-methyl-Histone H3-K79 antibody [RM2A93] (STJA0025067)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/ChIP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

Choose Options