Anti-SMAD antibodies | St John's Laboratory

Western blot analysis of extracts of C6 cells, using Smad1 antibody (STJ25602) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SMAD1 antibody (20-240) (STJ25602)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Smad1/9 (PTR2351) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HepG2Predicted band size: 52kDaObserved band size: 52kDa

Anti-SMAD1 antibody (400-465) [PTR2351] (STJA0006276)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot with Anti-Smad1 Antibody (STJ501648) with peptide on at 1:500 dilution in buffer.

Anti-Smad1 antibody (405-455) (STJ501648)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

D.melanogaster

£427.50

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Anti-Smad1 antibody (405-455) {Biotin} (STJ501649)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

D.melanogaster

£528.50

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Anti-Smad1 antibody (405-455) {FITC} (STJ501650)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

D.melanogaster

£528.50

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Western blot analysis of various cells using Smad1/5/9 Polyclonal Antibody diluted at 1：500

Anti-Smad1/5/9 antibody (10-59 aa) (STJ95690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from C2C12 cells using Smad1/Smad5/Smad9 Rabbit pAb (STJ11106932) at1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 15s.

Anti-Smad1/Smad5/Smad9 antibody (19-125 aa) (STJ11106932)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts from normal (control) and Smad2 knockout (KO) HeLa cells, using Smad2 antibody (STJ110012) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.

Anti-SMAD2 antibody (1-100) (STJ110012)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded human urothelial carcinoma using Smad2 Rabbit polyclonal antibody (STJ113167) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-SMAD2 antibody (1-100) (STJ113167)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g Smad2 antibody (STJ11101651). Western blot was performed from the immunoprecipitate using Smad2 antibody (STJ11101651) at a dilution of 1:1000.

Anti-SMAD2 antibody (1-100) [S1MR] (STJ11101651)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded human tonsil using Smad2 Rabbit polyclonal antibody (STJ11103803) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-SMAD2 antibody (175-270) (STJ11103803)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and SMAD2 knockout (KO) Hela (KO) cells, using [KO Validated] Smad2 Rabbit monoclonal antibody (STJ11104898) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SMAD2 antibody (367-467 aa) [S4898RM] (STJ11104898)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of Hela cell. 1, Smad2 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). MICU1 monoclonal antibody (Mix) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-SMAD2 antibody (418-467 aa) (STJ95694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/CoIP/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunocytochemistry analysis of Smad2 (green) in Hela using Smad2 antibody, and DAPI (blue).

Anti-SMAD2 antibody (81-100 aa) [R03-3F8] (STJA0013074)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Rat/Hamster

£264.50

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Western blot analysis using Smad2 monoclonal antibody against HEK293 (1) and SMAD2-hIgGFc transfected HEK293 (2) cell lysate.

Anti-SMAD2 antibody [5G7] (STJ98383)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot detection of Smad2 in Hela, A431, Jurkat and K562 cell lysates using Smad2 mouse mAb (1:500 diluted).Predicted band size:60KDa.Observed band size:60KDa.

Anti-SMAD2 antibody [6H5-E3-C11] (STJ99174)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

£144.00 - £348.00

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Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1, Smad2/3 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-Smad2/3 antibody (1-50 aa) (STJ95697)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey/Pig

£48.00 - £252.00

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Western blot analysis of various lysates using SMAD2/SMAD3 Rabbit pAb (STJ11107460) at 1:700 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-SMAD2/SMAD3 antibody (150-250 aa) (STJ11107460)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using SMAD2/SMAD3 Rabbit pAb (STJ11101471) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-SMAD2/SMAD3 antibody (150-270) (STJ11101471)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with SMAD2/SMAD3 using SMAD2/SMAD3 Rabbit pAb (STJ11106981) at1:1000 dilution incubated overnight at 4°C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>NC:293T cells transfected with SMAD5<br>Exposure time: 10s.

Anti-SMAD2/SMAD3 antibody (175-270 aa) (STJ11106981)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ChIP

Reactivity :

Human

£112.50 - £524.50

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Immunohistochemistry analysis of Smad3 in paraffin-embedded human tonsil using [KO Validated] Smad3 Rabbit monoclonal antibody (STJ11101652) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-SMAD3 antibody (100-200) [S2MR] (STJ11101652)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of Hela cell. 1, Smad3 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-actin monoclonal antibody (5B7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-SMAD3 antibody (145-194 aa) (STJ95699)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of rat ovary, using Smad3 antibody (STJ113806) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SMAD3 antibody (150-250) (STJ113806)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using Smad3 Rabbit polyclonal antibody (STJ11101192) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-SMAD3 antibody (150-425) (STJ11101192)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using Smad3 antibody (STJ113110) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SMAD3 antibody (190-425) (STJ113110)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunocytochemistry analysis of Smad3 (green) in A549 using Smad3 antibody, and DAPI (blue).

Anti-SMAD3 antibody (202-219 aa) [R09-7H4] (STJA0010795)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Rat

£246.50

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Immunohistochemical analysis of paraffin-embedded Human Liver Carcinoma Tissue using Smad3 Mouse mAb diluted at 1:200.

Anti-SMAD3 antibody (350-430 aa) [4C9] (STJ97772)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-SMAD4 (DPC4) antibody [B-8] (STJ16101862)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£1,484.50

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Human pancreatic ductal adenocarcinoma stained with anti-DPC4 (SMAD4) antibody using peroxidase-conjugate and DAB chromogen. Note absence of tumor cell staining while the non-tumor cells are positive.

Anti-SMAD4 antibody (1-552 aa) [B‐8] (STJ180086)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£162.50 - £583.50

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Immunohistochemistry of mouse Colon with Anti-SMAD4 primary antibody (STJ503059) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-SMAD4 antibody (131-181) (STJ503059)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-SMAD4 antibody (131-181) {Biotin} (STJ503063)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-SMAD4 antibody (131-181) {FITC} (STJ503064)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-SMAD4 antibody (136-156) {FITC} (STJ502007)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

£528.50

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Western blot analysis of lysates from wild type (WT) and Smad4 knockout (KO) HeLa cells, using [KO Validated] Smad4 Rabbit polyclonal antibody (STJ11100051) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SMAD4 antibody (160-450) (STJ11100051)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Immunofluorescence analysis of Hela cell. 1, Smad4 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GAPDH monoclonal antibody (2B8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-SMAD4 antibody (21-70 aa) (STJ95702)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Immunocytochemistry analysis of Smad4 (green) in Hela using Smad4 antibody, and DAPI (blue).

Anti-SMAD4 antibody (316-552 aa) [R05-1I7] (STJA0012592)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Rat

£264.50

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Western blot analysis of lysates from HCT116 cells, using Smad4 Rabbit pAb (STJ27624) at 1:600 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-SMAD4 antibody (320-552) (STJ27624)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g Smad4 rabbit monoclonal antibody (STJ11101653) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of Smad4 in representative gene loci (JUNB) , as shown in figure.

Anti-SMAD4 antibody (320-552) [S3MR] (STJ11101653)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA/ChIP/CUT&Tag

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunohistochemistry of mouse liver with Anti-SMAD4 primary antibody (STJ503060) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-SMAD4 antibody (400-450) (STJ503060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

£427.50

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Immunohistochemistry with Anti-SMAD4 Antibody (STJ503062) at 1:50 dilution in buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 20X magnification. FFPE section.

Anti-SMAD4 antibody (400-450) (STJ503062)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

D.melanogaster/Human/Monkey/Mouse/Rat

£427.50

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Anti-SMAD4 antibody (400-450) {Biotin} (STJ503065)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

£528.50

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Anti-SMAD4 antibody (400-450) {Biotin} (STJ503069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

£528.50

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Anti-SMAD4 antibody (400-450) {FITC} (STJ503066)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

£528.50

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Anti-SMAD4 antibody (400-450) {FITC} (STJ503070)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

£528.50

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Immunohistochemistry of mouse small intestine with Anti-SMAD4 primary antibody (STJ503061) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-SMAD4 antibody (502-552) (STJ503061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-SMAD4 antibody (502-552) {Biotin} (STJ503067)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-SMAD4 antibody (502-552) {FITC} (STJ503068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis using Smad4 monoclonal antibody against A431 (1) , SK-N-SH (2) , K562 (3) , HepG2 (4) and HUVE12 (5) cell lysate.

Anti-SMAD4 antibody [4G1C6] (STJ98385)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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