Anti-SMAD antibodies | St John's Laboratory

Anti-Phospho-Smad1 antibody (405-455) (STJ502549)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

D.melanogaster

£528.50

Choose Options

Anti-Phospho-Smad1 antibody (405-455) {Biotin} (STJ502550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

D.melanogaster

£584.50

Choose Options

Anti-Phospho-Smad1 antibody (405-455) {FITC} (STJ502551)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

D.melanogaster

£584.50

Choose Options

Western blot analysis of lysates from C6 cells, using Phospho-Smad1-S206 Rabbit pAb (STJ22396) at 1:1000 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-SMAD1-S206 antibody (STJ22396)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of lysates from HeLa cells using Phospho-Smad1-S465 Rabbit pAb (STJ11107625). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-Smad1-S465 antibody (STJ11107625)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of extracts of various cell lines, using Phospho-Smad2-S465/467 + Smad3-S423/425 antibody (STJ11105832) at 1:600 dilution. C6 cells were treated by TGF-Beta 3 (200 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-Smad2 + Smad3-S465/S467-S423/S425 antibody [S5832RM] (STJ11105832)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

Choose Options

Western blot analysis of various lysates, using Phospho-Smad2-S245/S250/S255 antibody (STJ11105827) at1:2000 dilution. 293T cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-Phospho-SMAD2-S245/S250/S255 antibody [S5827RM] (STJ11105827)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

Choose Options

Western blot analysis of various lysates using Phospho-Smad2-S250 Rabbit mAb (STJ11102595) at 1:1000 dilution. 293T cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight or treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. C2C12 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-SMAD2-S250 antibody [S5MR] (STJ11102595)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

Choose Options

Western blot analysis of lysates from HeLa cells, using Phospho-Smad2-S465/S467 Rabbit pAb (STJ11101059) at 1:1000 dilution. HeLa cells were treated by TGF-Beta (10ng/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-SMAD2-S465/S467 antibody (STJ11101059)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

Choose Options

Western blot analysis of extracts of various lysates, using Phospho-Smad2-S465/S467 antibody (STJ11105831) at1:2000 dilution. HeLa and NIH/3T3 were treated by TGF-Beta 3 (H) (200 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-SMAD2-S465/S467 antibody [S5831RM] (STJ11105831)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

Choose Options

Western blot analysis of lysates from HeLa cells, using Phospho-Smad2-S467 Rabbit pAb (STJ22397) at 1:1000 dilution. HeLa cells were treated by TGF-Beta (10 ng/ml) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-SMAD2-S467 antibody (STJ22397)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of lysates from HeLa cells using Phospho-Smad2-T220 Rabbit pAb (STJ11101044) at 1:500 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 10 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 30 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-SMAD2-T220 antibody (STJ11101044)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of lysates from C2C12 cells, using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (STJ114840) at 1:800 dilution. C2C12 cells were treated by TGF-Beta (10 ng/ml) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-SMAD2/SMAD3-S423/425 antibody (STJ114840)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of various lysates using (STJ11103777) at 1:1000 dilution. Hela cells were treated by TGF-Beta (10 ng/ml) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-Smad3-S423/S425 antibody (STJ11103777)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of lysates from C6 cells, using Phospho-Smad3-S423/S425 Rabbit mAb (STJ11102566) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 10 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-Smad3-S423/S425 antibody [S6MR] (STJ11102566)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

Choose Options

Western blot analysis of lysates from HeLa cells, using Phospho-Smad3-T179 Rabbit pAb (STJ114841). HeLa cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-SMAD3-T179 antibody (STJ114841)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Anti-Phospho-SMAD5-S463/465 antibody [S5792RM] (STJ11105792)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded rat kidney using Phospho-Smad5-S463/S465 Rabbit polyclonal antibody (STJ11105818) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-SMAD5-S463/S465 antibody (STJ11105818)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of lysates from HeLa cells, using Phospho-Smad5-S463/S465 Rabbit mAb (STJ11102605) at 1:1000 dilution or Smad5 antibody (STJ25609). Hela cells were treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-SMAD5-S463/S465 antibody [S5MR] (STJ11102605)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

Choose Options

Western blot analysis of C6, using Phospho-Smad5-S465 Rabbit monoclonal antibody (STJ11105872) at 1:2000 dilution. C6 cells were treated by MG132 (50 Mu M) and TGF Beta at 37 °C for 90 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-SMAD5-S465 antibody [S5872RM] (STJ11105872)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£146.50 - £642.50

Choose Options

Anti-Phospho-SMAD9-Y92 antibody [S5897RM] (STJ11105897)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

Choose Options

Immunofluorescence analysis of NIH/3T3 cells using Smad1 Rabbit polyclonal antibody (STJ11104508) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-SMAD1 antibody (20-240) (STJ11104508)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of extracts of C6 cells, using Smad1 antibody (STJ25602) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SMAD1 antibody (20-240) (STJ25602)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot with Anti-Smad1 Antibody (STJ501648) with peptide on at 1:500 dilution in buffer.

Anti-Smad1 antibody (405-455) (STJ501648)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

D.melanogaster

£427.50

Choose Options

Anti-Smad1 antibody (405-455) {Biotin} (STJ501649)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

D.melanogaster

£528.50

Choose Options

Anti-Smad1 antibody (405-455) {FITC} (STJ501650)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

D.melanogaster

£528.50

Choose Options

Western blot analysis of lysates from C2C12 cells using Smad1/Smad5/Smad9 Rabbit pAb (STJ11106932) at1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 15s.

Anti-Smad1/Smad5/Smad9 antibody (19-125 aa) (STJ11106932)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

Choose Options

Western blot analysis of extracts from normal (control) and Smad2 knockout (KO) HeLa cells, using Smad2 antibody (STJ110012) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.

Anti-SMAD2 antibody (1-100) (STJ110012)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded human urothelial carcinoma using Smad2 Rabbit polyclonal antibody (STJ113167) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-SMAD2 antibody (1-100) (STJ113167)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g Smad2 antibody (STJ11101651). Western blot was performed from the immunoprecipitate using Smad2 antibody (STJ11101651) at a dilution of 1:1000.

Anti-SMAD2 antibody (1-100) [S1MR] (STJ11101651)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded human tonsil using Smad2 Rabbit polyclonal antibody (STJ11103803) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-SMAD2 antibody (175-270) (STJ11103803)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

Choose Options

Western blot analysis of lysates from wild type (WT) and SMAD2 knockout (KO) Hela (KO) cells, using [KO Validated] Smad2 Rabbit monoclonal antibody (STJ11104898) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SMAD2 antibody (367-467 aa) [S4898RM] (STJ11104898)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

Choose Options

Immunocytochemistry analysis of Smad2 (green) in Hela using Smad2 antibody, and DAPI (blue).

Anti-SMAD2 antibody (81-100 aa) [R03-3F8] (STJA0013074)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Rat/Hamster

£264.50

Choose Options

Western blot analysis of various lysates using SMAD2/SMAD3 Rabbit pAb (STJ11107460) at 1:700 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-SMAD2/SMAD3 antibody (150-250 aa) (STJ11107460)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of various lysates using SMAD2/SMAD3 Rabbit pAb (STJ11101471) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-SMAD2/SMAD3 antibody (150-270) (STJ11101471)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with SMAD2/SMAD3 using SMAD2/SMAD3 Rabbit pAb (STJ11106981) at1:1000 dilution incubated overnight at 4°C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>NC:293T cells transfected with SMAD5<br>Exposure time: 10s.

Anti-SMAD2/SMAD3 antibody (175-270 aa) (STJ11106981)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ChIP

Reactivity :

Human

£112.50 - £524.50

Choose Options

Immunohistochemistry analysis of Smad3 in paraffin-embedded human tonsil using [KO Validated] Smad3 Rabbit monoclonal antibody (STJ11101652) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-SMAD3 antibody (100-200) [S2MR] (STJ11101652)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

Choose Options

Western blot analysis of extracts of rat ovary, using Smad3 antibody (STJ113806) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SMAD3 antibody (150-250) (STJ113806)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£112.50 - £524.50

Choose Options

Immunofluorescence analysis of NIH/3T3 cells using Smad3 Rabbit polyclonal antibody (STJ11101192) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-SMAD3 antibody (150-425) (STJ11101192)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of various lysates, using Smad3 antibody (STJ113110) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SMAD3 antibody (190-425) (STJ113110)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Immunocytochemistry analysis of Smad3 (green) in A549 using Smad3 antibody, and DAPI (blue).

Anti-SMAD3 antibody (202-219 aa) [R09-7H4] (STJA0010795)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Rat

£246.50

Choose Options

Anti-SMAD4 (DPC4) antibody [B-8] (STJ16101862)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£1,484.50

Choose Options

Human pancreatic ductal adenocarcinoma stained with anti-DPC4 (SMAD4) antibody using peroxidase-conjugate and DAB chromogen. Note absence of tumor cell staining while the non-tumor cells are positive.

Anti-SMAD4 antibody (1-552 aa) [B‐8] (STJ180086)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£162.50 - £583.50

Choose Options

Immunohistochemistry of mouse Colon with Anti-SMAD4 primary antibody (STJ503059) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-SMAD4 antibody (131-181) (STJ503059)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

Choose Options

Anti-SMAD4 antibody (131-181) {Biotin} (STJ503063)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

Choose Options

Anti-SMAD4 antibody (131-181) {FITC} (STJ503064)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

Choose Options

Anti-SMAD4 antibody (136-156) {FITC} (STJ502007)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

£528.50

Choose Options

Western blot analysis of lysates from wild type (WT) and Smad4 knockout (KO) HeLa cells, using [KO Validated] Smad4 Rabbit polyclonal antibody (STJ11100051) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SMAD4 antibody (160-450) (STJ11100051)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

Choose Options