Anti-CDK1 antibodies

Immunohistochemistry analysis of paraffin-embedded mouse brain using CDK1 antibody (STJ114288) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CDK1 antibody (1-297) (STJ114288)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using Cdk1/Cdc2 Polyclonal Antibody diluted at 1：2000

Anti-CDK1 antibody (101-150 aa) (STJ92194)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Cdc2 Polyclonal Antibody diluted at 1：1000

Anti-CDK1 antibody (131-180 aa) (STJ92155)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunoprecipitation analysis of 600 Mu g extracts of C6 cells using 3 Mu g CDK1 antibody (STJ11104500). Western blot was performed from the immunoprecipitate using CDK1 antibody (STJ11104500) at a dilution of 1:1000.

Anti-CDK1 antibody (198-297) (STJ11104500)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of CDK1 in paraffin-embedded mouse brain using CDK1 Rabbit polyclonal antibody (STJ23048) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CDK1 antibody (198-297) (STJ23048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using CDK1 rabbit monoclonal antibody (STJ11101328) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CDK1 antibody (198-297) [S8MR] (STJ11101328)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Immunofluorescence analysis of human-stomach tissue. 1, Cdc2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-CDK1 antibody (5-54 aa) (STJ92154)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-CDK1 antibody (N-Term) [M226] (STJA0005340)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC/IP

Reactivity :

Human/Mouse/Rat

£319.50

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Western blot detection of CDC2/CDK1 in K562, A549, Jurkat and Hela cell lysates using CDC2/CDK1 mouse mAb (1:100 diluted).Predicted band size: 34KDa.Observed band size: 34KDa.

Anti-CDK1 antibody [3B9-F8-B12] (STJ99114)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis using Cdk1 monoclonal antibody against HeLa (1) , Jurkat (2) , K562 (3) , A431 (4) , MCF-7 (5) , RAW264.7 (6) , NIN/3T3 (7) , PC-12 (8) , and Cos7 (9) cell lysate.

Anti-CDK1 antibody [5A6] (STJ97946)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£144.00 - £348.00

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Western blot analysis using CDC2 monoclonal antibody against Jurkat (1) cell lysate.

Anti-CDK1 antibody [8C5A7F10] (STJ97943)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of various cells using Cdk1/2/3 Polyclonal Antibody

Anti-Cdk1/2/3 antibody (1-50 aa) (STJ92193)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Cdk1/p34cdc2 antibody [POH-1] (STJ16101079)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Cow/Human/Mink/Monkey

£287.50 - £1,324.50

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Western blot of human T47D cells showing specific immunolabeling of the ~34 kDa cdc2 phosphorylated at Tyr15 (Control, Lane 1). Treatment with EGF (30 ng per ml for 30 min) caused dephosphorylation of the Tyr15 on cdc2 (Lane 2). Phosphospecificity is shown in lanes 3 and 4, where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase, Lambda-Ptase (1200 units for 30 min).

Anti-Phospho-cdc2-Tyr15 antibody (STJA0003591)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Xenopus/Rat/Zebrafish

£454.50

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Western blot analysis of lysates from HeLa cells, using Phospho-CDK1-T14 Rabbit pAb (STJ22058) at 1:500 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-CDK1-T14 antibody (STJ22058)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells using Phospho-CDK1-T14 Rabbit monoclonal antibody (STJ11105900) at 1:3000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-CDK1-T14 antibody [S5900RM] (STJ11105900)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-CDK1-T161 Rabbit pAb (STJ22061) at 1:400 dilution. HeLa cells were treated by EGF. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-CDK1-T161 antibody (STJ22061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-CDK1-Thr14 antibody (STJ194705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-CDK1-Thr161 antibody (126-175 aa) (STJ91199)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Cdc2 (T161) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-CDK1-Thr161 antibody (131-180 aa) (STJ90213)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-CDK1-Tyr-15 antibody [M231] (STJA0005341)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Western blot analysis of Cdc2 (phospho Tyr15) Polyclonal Antibody, using Hela, C6 cell treated or untreated with forskolin 40nM 30', Nocodazole 0.1ug/ml 18hour, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-CDK1-Tyr15 antibody (5-54 aa) (STJ90214)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using Phospho-CDK1-Y15 Rabbit pAb (STJ23048). HeLa cells were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours or Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-CDK1-Y15 antibody (STJ22059)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates, using Phospho-CDK1-Y15 antibody (STJ11105835) at1:2000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-Phospho-CDK1-Y15 antibody [S5835RM] (STJ11105835)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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CDK1 antibodies

Target Information

Synonyms

Anti-CDK1 antibody (1-297) (STJ114288)

Anti-CDK1 antibody (101-150 aa) (STJ92194)

Anti-CDK1 antibody (131-180 aa) (STJ92155)

Anti-CDK1 antibody (198-297) (STJ11104500)

Anti-CDK1 antibody (198-297) (STJ23048)

Anti-CDK1 antibody (198-297) [S8MR] (STJ11101328)

Anti-CDK1 antibody (5-54 aa) (STJ92154)

Anti-CDK1 antibody (N-Term) [M226] (STJA0005340)

Anti-CDK1 antibody [3B9-F8-B12] (STJ99114)

Anti-CDK1 antibody [5A6] (STJ97946)

Anti-CDK1 antibody [8C5A7F10] (STJ97943)

Anti-Cdk1/2/3 antibody (1-50 aa) (STJ92193)

Anti-Cdk1/p34cdc2 antibody [POH-1] (STJ16101079)

Anti-Phospho-cdc2-Tyr15 antibody (STJA0003591)

Anti-Phospho-CDK1-T14 antibody (STJ22058)

Anti-Phospho-CDK1-T14 antibody [S5900RM] (STJ11105900)

Anti-Phospho-CDK1-T161 antibody (STJ22061)

Anti-Phospho-CDK1-Thr14 antibody (STJ194705)

Anti-Phospho-CDK1-Thr161 antibody (126-175 aa) (STJ91199)

Anti-Phospho-CDK1-Thr161 antibody (131-180 aa) (STJ90213)

Anti-Phospho-CDK1-Tyr-15 antibody [M231] (STJA0005341)

Anti-Phospho-CDK1-Tyr15 antibody (5-54 aa) (STJ90214)

Anti-Phospho-CDK1-Y15 antibody (STJ22059)

Anti-Phospho-CDK1-Y15 antibody [S5835RM] (STJ11105835)