Immunofluorescence Antibodies | St John's Laboratory

Immunofluorescence analysis of Human liver tissue using Complement factor H Rabbit polyclonal antibody (STJ115641) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining.

Anti-CFH antibody (1-240) (STJ115641)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HepG2 cells using Complement factor H Rabbit polyclonal antibody (STJ23105) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CFH antibody (19-323) (STJ23105)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of Rat liver, using CFHR3 rabbit polyclonal antibody (STJ110086) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CFHR3 antibody (80-200) (STJ110086)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using Cofilin Polyclonal Antibody diluted at 1：500

Anti-CFL1 antibody (1-50 aa) (STJ92371)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry of mouse kidney with Anti-CFL1 primary antibody (STJ500501) at 1:100 dilution in Buffer. Magnification is at 40X.

Anti-CFL1 antibody (120-166) (STJ500501)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-CFL1 antibody (120-166) {Biotin} (STJ500502)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-CFL1 antibody (120-166) {FITC} (STJ500503)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of various lysates using CFL2 Rabbit pAb (STJ110316) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-CFL2 antibody (1-166) (STJ110316)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-CFLAR antibody (181-230 aa) (STJ92242)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of U-2 OS cells using CFP Rabbit polyclonal antibody (STJ27351) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CFP antibody (190-469) (STJ27351)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CFP antibody (STJ140287)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

GFP

£135.50 - £394.50

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Western blot analysis of various lysates using CFTR Rabbit pAb (STJ110684) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-CFTR antibody (700-840) (STJ110684)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using CFTR Polyclonal Antibody diluted at 1：2000

Anti-CFTR antibody (711-760 aa) (STJ92253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Confocal imaging of HT-29 cells using cGAS Rabbit pAb (STJ11106791, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse mAb (STJ11107556, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody (STJS001209, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-cGAS antibody (1-100 aa) (STJ11106791)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-cGAS antibody (1-501 aa) [SRM] (STJ11107519)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from C2C12 cells, using cGAS Rabbit pAb (STJ11106960) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-cGAS antibody (400-500 aa) (STJ11106960)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-CGGBP1 antibody (41-90 aa) (STJ92254)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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STJ71112 (0. 2µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CGI58/ABHD5 antibody (Internal) (STJ71112)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/IHC

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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Western blot analysis of 293 cells using CGREF1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CGREF1 antibody (1-50 aa) (STJ96741)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of A549 cells using CGREF1 Polyclonal Antibody

Anti-CGREF1 antibody (241-290 aa) (STJ92258)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of paraffin-embedded Human pancreas tissue using CHAC1 Rabbit polyclonal antibody (STJ118037) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-CHAC1 antibody (1-222) (STJ118037)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-CHAF1B antibody (71-120 aa) (STJ91970)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of Hela cells using CHAMP1 Rabbit polyclonal antibody (STJ113290) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CHAMP1 antibody (563-812) (STJ113290)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of THP-1 cells using CHAT antibody (STJ115210) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CHAT antibody (331-630) (STJ115210)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CHAT antibody (461-560 aa) [SRM] (STJ11107317)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunocytochemistry analysis of Choline Acetyltransferase (green) in SH-SY5Y using Choline Acetyltransferase antibody, and DAPI (blue).

Anti-CHAT antibody (735-748 aa) [R07-1G8] (STJA0011140)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Mouse/Rat

£264.50

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Western blot analysis of extracts of various cell lines, using CHCHD2 antibody (STJ119068) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CHCHD2 antibody (75-145) (STJ119068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using CHCHD3 Rabbit pAb (STJ111317) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 40s.

Anti-CHCHD3 antibody (1-227) (STJ111317)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of L929 cells using CHCHD7 Rabbit polyclonal antibody (STJ11100443) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CHCHD7 antibody (1-97) (STJ11100443)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells using CHD2 antibody (STJ115437). Blue: DAPI for nuclear staining.

Anti-CHD2 antibody (242-501) (STJ115437)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using CHD3 rabbit monoclonal antibody (STJ11102316) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CHD3 antibody (1-100) [S6MR] (STJ11102316)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis using Mi2-Alpha monoclonal antibody against Y7P, Raji cell lysate.

Anti-CHD3 antibody (C-Term) (STJ98518)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Cow/Dog/Pig

£144.00 - £348.00

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Western blot analysis using Mi2-Alpha monoclonal antibody against HeLa (1) , K562 (2) , Jurkat (3) , NTERA-2 (4) , HEK293 (5) , Raji (6) cell lysate and mouse brain (7) tissue lysate.

Anti-CHD3 antibody [2G4] (STJ98244)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse

£144.00 - £348.00

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Immunofluorescence analysis of U20S cells using CHD4 antibody (STJ113182) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CHD4 antibody (1520-1690) (STJ113182)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HepG2 cells, using CHD4 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-CHD4 antibody (571-620 aa) (STJ94119)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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STJ71152 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-CHD5 antibody (Internal) (STJ71152)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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Anti-CHD6 antibody (STJ190697)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-CHD7 antibody (1703-1753 aa) (STJ196604)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-CHD8 antibody (STJ190698)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-CHD9 antibody (STJ190699)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of PC-12 cells using Chk1 Rabbit polyclonal antibody (STJ29967) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

Anti-CHEK1 antibody (148-307) (STJ29967)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of MCF7 cells using Chk1 Polyclonal Antibody

Anti-CHEK1 antibody (286-335 aa) (STJ92267)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunoprecipitation analysis of 200 Mu g extracts of MCF-7 cells using 1 Mu g Chk2 antibody (STJ114888). Western blot was performed from the immunoprecipitate using Chk2 antibody (STJ114888) at a dilution of 1:1000.

Anti-CHEK2 antibody (1-220) (STJ114888)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of Chk2 Polyclonal Antibody, using Hela, HCT116, SW480, HEK293 cell, 4°C over night, secondary antibody (cat： (NA was diluted at 1:10000, 37°C 1hour.

Anti-CHEK2 antibody (35-84 aa) (STJ92273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunocytochemistry analysis of Chk2 (green) in HCT116 using Chk2 antibody, and DAPI (blue).

Anti-CHEK2 antibody (43-167 aa) [R06-1B4] (STJA0011138)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

£246.50

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Western blot analysis using Chk2 monoclonal antibody against cell lysate from various cell types.

Anti-CHEK2 antibody (481-531 aa) [1C12B8] (STJ97956)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot with Anti-CHERP Antibody (STJ500534) at 1 pg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 ug.

Anti-CHERP antibody (846-913) (STJ500534)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Monkey/Mouse/Rat

£427.50

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Anti-CHERP antibody (846-913) {Biotin} (STJ500535)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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