Immunofluorescence Antibodies | St John's Laboratory

Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, CK16 monoclonal antibody (6F6) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-KRT16 antibody [6F6] (STJ96958)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using Cytokeratin 17 (KRT17) antibody (STJ114865) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-KRT17 antibody (133-432) (STJ114865)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Confocal immunofluorescence analysis of HeLa cells using Cytokeratin 17 (Cytokeratin 17 (KRT17) ) antibody (STJ24351) at dilution of 1:200. Blue: DAPI for nuclear staining.

Anti-KRT17 antibody (133-432) (STJ24351)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Cytokeratin 17 antibody. The HRP-conjugated anti- mouseIgG antibody was used to detect the antibody. Predicted band size: 48 kDa

Anti-KRT17 antibody (300-432 aa) [ABT-CK17] (STJ196821)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Western blot analysis of various cells using Cytokeratin 17 Polyclonal Antibody diluted at 1：2000

Anti-KRT17 antibody (381-430 aa) (STJ92630)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, CK17 monoclonal antibody (10A1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-KRT17 antibody [10A1] (STJ96983)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis using Cytokeratin 17 monoclonal antibody against HeLa (1) , MCF-7 (2) and A431 (3) cell lysate.

Anti-KRT17 antibody [3B12] (STJ97983)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-KRT17 antibody [ABT164R] (STJ197052)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Immunofluorescence analysis of rat-lung tissue. 1, Cytokeratin 18 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-KRT18 antibody (1-50 aa) (STJ92631)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IP/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of HepG2 cells using Cytokeratin 18 (KRT18) Rabbit polyclonal antibody (STJ116405) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-KRT18 antibody (127-430) (STJ116405)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis using Cytokeratin 18 monoclonal antibody against HeLa (1) , NIH/3T3 (2) , A549 (3) , Jurkat (4) , MCF-7 (5) , HepG2 (6) , A431 (7) , HEK293 (8) and K562 (9) cell lysate.

Anti-KRT18 antibody (391-483 aa) [4D11E4] (STJ97984)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-KRT18 antibody [ABT165R] (STJ197076)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Immunofluorescence analysis of MCF7 cells using Cytokeratin 19 (KRT19) Rabbit monoclonal antibody (STJ11101578) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-KRT19 antibody (1-100) [S8MR] (STJ11101578)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£146.50 - £642.50

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Anti-KRT19 antibody (200-300 aa) [PT0087] (STJ196823)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£174.00 - £252.00

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Immunofluorescence analysis of MCF7 cells using Cytokeratin 19 (KRT19) Rabbit polyclonal antibody (STJ24355) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-KRT19 antibody (210-400) (STJ24355)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of human-liver tissue. 1, Cytokeratin 19 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-KRT19 antibody (231-280 aa) (STJ92634)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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ACHN whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CK19 (ABT050) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: ACHNPredicted band size: 44kDaObserved band size: 44kDa

Anti-KRT19 antibody (300-400 aa) [ABT050] (STJ197143)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£174.00 - £252.00

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Western blot analysis of A549 cells using Cytokeratin 19 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-KRT19 antibody (321-370 aa) (STJ96714)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis using Cytokeratin 19 monoclonal antibody against truncated KRT19-His recombinant protein (1) and full-length KRT19 (aa1-400) -hIgGFc transfected CHO-K1 cell lysate (2).

Anti-KRT19 antibody (80-400 aa) [9H8G6] (STJ97985)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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IHC staining of human colon cancer tissue, diluted at 1:200.

Anti-KRT19 antibody [11F5] (STJ96953)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using Cytokeratin 19 monoclonal antibody against T47D (1) , MCF-7 (2) , SKBR-3 (3) , HepG2 (4) , Caco-2 (5) and SW620 (6) cell lysate.

Anti-KRT19 antibody [1H6] (STJ97987)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis using Cytokeratin 19 monoclonal antibody against T47D (1) , MCF-7 (2) , HepG2 (3) and SW620 (4) cell lysate.

Anti-KRT19 antibody [4E8] (STJ97988)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-KRT19 antibody [ABT166R] (STJ197060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Immunofluorescence analysis of paraffin-embedded Human Colon using Cytokeratin 20 (KRT20) Rabbit polyclonal antibody (STJ11104411) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-KRT20 antibody (245-424) (STJ11104411)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and Cytokeratin 20 (Cytokeratin 20 (KRT20) ) knockout (KO) HeLa cells, using [KO Validated] Cytokeratin 20 (KRT20) Rabbit polyclonal antibody (STJ119970) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-KRT20 antibody (245-424) (STJ119970)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Confocal immunofluorescence analysis of A-431 cells using Cytokeratin 20 (Cytokeratin 20 (KRT20) ) antibody (STJ24356) at dilution of 1:200. Blue: DAPI for nuclear staining.

Anti-KRT20 antibody (245-424) (STJ24356)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-KRT20 antibody (300-424 aa) [ABT-Ck20] (STJ196757)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£174.00 - £252.00

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SW480 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CK20 (ABT044) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: SW480Predicted band size: 48kDaObserved band size: 48kDa

Anti-KRT20 antibody (300-424 aa) [ABT044] (STJ197146)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Western blot analysis of various cells using Cytokeratin 20 Polyclonal Antibody diluted at 1：500

Anti-KRT20 antibody (375-424 aa) (STJ92635)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-KRT20 antibody [ABT24R] (STJ197067)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Western blot analysis of extracts of H460 cells, using KRT3 Antibody (STJ112157) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-KRT3 antibody (230-390) (STJ112157)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Confocal imaging of human tonsil using Cytokeratin 4 (KRT4) Rabbit monoclonal antibody (STJ11101221, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol.

Anti-KRT4 antibody (1-100) [S1MR] (STJ11101221)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunoprecipitation analysis of 200 Mu g extracts of A-431 cells using 3 Mu g Cytokeratin 5 (Cytokeratin 5 (KRT5) ) antibody (STJ24360). Western blot was performed from the immunoprecipitate using Cytokeratin 5 (Cytokeratin 5 (KRT5) ) antibody (STJ24360) at a dilution of 1:5000.

Anti-KRT5 antibody (1-100) (STJ24360)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts of A-431 cells, using Cytokeratin 5 (KRT5) rabbit monoclonal antibody (STJ11101321) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-KRT5 antibody (491-590) [S1MR] (STJ11101321)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of various cells using Cytokeratin 5 Polyclonal Antibody diluted at 1：1000

Anti-KRT5 antibody (541-590 aa) (STJ92636)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using Cytokeratin 5 monoclonal antibody against truncated CK5 recombinant protein.

Anti-KRT5 antibody [3E2F1] (STJ97989)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-KRT5 antibody [ABT168R] (STJ197080)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Western blot analysis of extracts of various cell lines, using Cytokeratin 7 (KRT7) (KRT7) rabbit monoclonal antibody (STJ11102150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-KRT7 antibody (1-100) [S0MR] (STJ11102150)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of rat bronchus using Cytokeratin 7 (KRT7) Rabbit polyclonal antibody (STJ118022) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-KRT7 antibody (1-94) (STJ118022)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of rat bronchus cells using Cytokeratin 7 (Cytokeratin 7 (KRT7) ) antibody (STJ24364) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-KRT7 antibody (230-469) (STJ24364)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Cytokeratin 7 (KRT7) (KRT7) rabbit monoclonal antibody (STJ11102055) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-KRT7 antibody (291-463) [S5MR] (STJ11102055)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of various cells using Cytokeratin 7 Polyclonal Antibody diluted at 1：1000

Anti-KRT7 antibody (420-469 aa) (STJ92637)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis using Cytokeratin 7 monoclonal antibody against T47D, HeLa cell lysate.

Anti-KRT7 antibody (C-Term) (STJ98484)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat/Dog

£144.00 - £348.00

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Western blot analysis using Cytokeratin 7 monoclonal antibody against A459, HeLa cell lysate.

Anti-KRT7 antibody (N-Term) (STJ98483)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat/Cow

£144.00 - £348.00

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Immunofluorescence analysis of Hela cell. 1, FoxO1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). CK7 monoclonal antibody (12D7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-KRT7 antibody [12D7] (STJ96956)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC/IP

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-KRT77 antibody (STJ193199)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of MCF7 cells using Cytokeratin 8 (KRT8) Rabbit polyclonal antibody (STJ24365) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-KRT8 antibody (1-483) (STJ24365)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis using Cytokeratin 8 monoclonal antibody against A549 (1) , HeLa (2) , MCF-7 (3) , A431 (4) and HepG2 (5) cell lysate.

Anti-KRT8 antibody (391-483 aa) [8A5D12] (STJ97992)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

Choose Options