| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
PRAS40 Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:500 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Tissue Specificity | Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293). |
| Post Translational Modifications | Phosphorylated by AKT1.phosphorylation takes place in response to insulin treatment and promotes AKT1S1 interaction with 14-3-3 proteins, leading to relieve its inhibitor activity. Phosphorylated by MTOR following mTORC1 activation, inhibiting AKT1S1 inhibitor activity: phosphorylation by MTOR probably serves as a feedback loop that relieves inhibition from AKT1S1 in response to mTORC1 inactivation. Phosphorylation at Thr-246 by DYRK3 relieves inhibitory function on mTORC1. |
| Function | Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. In absence of insulin and nutrients, AKT1S1 associates with the mTORC1 complex and directly inhibits mTORC1 activity by blocking the MTOR substrate-recruitment site. In response to insulin and nutrients, AKT1S1 dissociates from mTORC1. Its activity is dependent on its phosphorylation state and binding to 14-3-3. May also play a role in nerve growth factor-mediated neuroprotection. |
| Peptide Name | Proline-Rich Akt1 Substrate 140 Kda Proline-Rich Akt Substrate |
| Database Links | Reactome: R-HSA-165159Reactome: R-HSA-166208Reactome: R-HSA-198323Reactome: R-HSA-3371571Reactome: R-HSA-5674400 |
| Cellular Localisation | CytoplasmCytosolFound In The Cytosolic Fraction Of The Brain |
| Alternative Peptide Names | Proline-Rich Akt1 Substrate 1 protein40 Kda Proline-Rich Akt Substrate proteinAKT1S1 proteinPRAS40 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
