| Applications: | Immunodepletion/Immunocompetition |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | PDE4A8 Blocking Peptide is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: | Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | PDE4A |
| Gene ID: | 5141 |
| Uniprot ID: | PDE4A_HUMAN |
| Immunogen: | Synthetic peptide specific only for PDE4A8 protein. |
| Tissue Specificity | Isoform 1: Expressed in lymphoid cell subsets including CD8-positive T cells and T-helper 2 cells. Expressed in dendritic cells. Isoform 2: Highly expressed in liver, stomach, testis, thyroid and adrenal glands and at a lower extent in placenta, kidney, pancreas, ovary, uterus and skin. Expressed in myeloid cell subsets including dendritic cells, monocytes, macrophages, eosinophils and mast cells. Expressed in natural killer cells. Expressed in bronchial smooth muscle. Isoform 6: Expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7: Expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Found in specific neuronal subpopulations including cortical pyramidal neurons, horn neurons in the spinal cord and Purkinje cells in cerebellum (at protein level). |
| Post Translational Modifications | Isoform 1: Proteolytically cleaved by CASP3. Isoform 2: Phosphorylated at Ser-119 by PKA. |
| Function | Hydrolyzes the second messenger 3',5'-cyclic AMP (cAMP), which is a key regulator of many important physiological processes. Isoform 1: Efficiently hydrolyzes cAMP. Isoform 2: Efficiently hydrolyzes cAMP. Isoform 3: Efficiently hydrolyzes cAMP. The phosphodiesterase activity is not affected by calcium, calmodulin or cyclic GMP (cGMP) levels. Does not hydrolyze cGMP. Isoform 4: Efficiently hydrolyzes cAMP. Isoform 6: Efficiently hydrolyzes cAMP. Isoform 7: Efficiently hydrolyzes cAMP. |
| Peptide Name | 3' -5'-Cyclic-Amp Phosphodiesterase 4a Dpde2 Pde46 Camp-Specific Phosphodiesterase 4a |
| Database Links | Reactome: R-HSA-180024 Reactome: R-HSA-418555 |
| Cellular Localisation | Isoform 1: Cytoplasm Perinuclear Region Isoform 2: Cytoplasm Cell Projection Ruffle Membrane Isoform 3: Cytoplasm Cytosol Isoform 4: Membrane Peripheral Membrane Protein Isoform 4 Has Propensity For Association With Membranes Isoform 6: Cytoplasm Isoform 7: Cytoplasm Membrane Predominantly Cytosolic |
| Alternative Peptide Names | 3' -5'-Cyclic-Amp Phosphodiesterase 4a protein Dpde2 protein Pde46 protein Camp-Specific Phosphodiesterase 4a protein PDE4A protein DPDE2 protein |
Information sourced from Uniprot.org