| Applications: | Immunodepletion/Immunocompetition |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Pannexin 1 Blocking Peptide is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: | Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | PANX1 |
| Gene ID: | 24145 |
| Uniprot ID: | PANX1_HUMAN |
| Immunogen: | Synthetic peptide taken within amino acid region from human Pannexin 1 protein |
| Tissue Specificity | Widely expressed. Highest expression is observed in oocytes and brain. Detected at very low levels in sperm cells. |
| Post Translational Modifications | S-nitrosylation inhibits channel currents and ATP release. N-glycosylation plays a role in cell surface targeting. Glycosylation at its extracellular surface makes unlikely that two oligomers could dock to form an intercellular channel such as in gap junctions. Exists in three glycosylation states: non-glycosylated (GLY0), high-mannose glycosylated (GLY1), and fully mature glycosylated (GLY2). Cleaved by CASP3 and CASP7 during apoptosis. Cleavage opens the channel for the release of metabolites and induces plasma membrane permeability during apoptosis. Phosphorylated at Tyr-199 by SRC. Phosphorylation activates ATP release. Constitutively phosphorylated in vascular smooth muscle cells. |
| Function | Ion channel involved in a variety of physiological functions such as blood pressure regulation, apoptotic cell clearance and oogenesis. Forms anion-selective channels with relatively low conductance and an order of permeabilities: nitrate>iodide>chlroride>>aspartate=glutamate=gluconate. Can release ATP upon activation through phosphorylation or cleavage at C-terminus. May play a role as a Ca(2+)-leak channel to regulate ER Ca(2+) homeostasis. Caspase-activated pannexin-1: During apoptosis, the C terminal tail is cleaved by caspases, which opens the main pore acting as a large-pore ATP efflux channel with a broad distribution, which allows the regulated release of molecules and ions smaller than 1 kDa, such as nucleotides ATP and UTP, and selective plasma membrane permeability to attract phagocytes that engulf the dying cells. |
| Peptide Name | Pannexin-1 Panx1 Cleaved Into - Caspase-Activated Pannexin-1 Caspase-Activated Panx1 |
| Database Links | Reactome: R-HSA-112303 Reactome: R-HSA-844456 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Endoplasmic Reticulum Membrane |
| Alternative Peptide Names | Pannexin-1 protein Panx1 Cleaved Into - Caspase-Activated Pannexin-1 protein Caspase-Activated Panx1 protein PANX1 protein MRS1 protein UNQ2529 protein PRO6028 protein |
Information sourced from Uniprot.org