| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Npas4 Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:500 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Tissue Specificity | Brain. |
| Post Translational Modifications | Ubiquitinated, leading to degradation by the proteosome. |
| Function | Transcription factor expressed in neurons of the brain that regulates the excitatory-inhibitory balance within neural circuits and is required for contextual memory in the hippocampus. Plays a key role in the structural and functional plasticity of neurons. Acts as an early-response transcription factor in both excitatory and inhibitory neurons, where it induces distinct but overlapping sets of late-response genes in these two types of neurons, allowing the synapses that form on inhibitory and excitatory neurons to be modified by neuronal activity in a manner specific to their function within a circuit, thereby facilitating appropriate circuit responses to sensory experience. In excitatory neurons, activates transcription of BDNF, which in turn controls the number of GABA-releasing synapses that form on excitatory neurons, thereby promoting an increased number of inhibitory synapses on excitatory neurons. In inhibitory neurons, regulates a distinct set of target genes that serve to increase excitatory input onto somatostatin neurons, probably resulting in enhanced feedback inhibition within cortical circuits. The excitatory and inhibitory balance in neurons affects a number of processes, such as short-term and long-term memory, acquisition of experience, fear memory, response to stress and social behavior. Acts as a regulator of dendritic spine development in olfactory bulb granule cells in a sensory-experience-dependent manner by regulating expression of MDM2. Efficient DNA binding requires dimerization with another bHLH protein, such as ARNT, ARNT2 or BMAL1. Can activate the CME (CNS midline enhancer) element. |
| Peptide Name | Neuronal Pas Domain-Containing Protein 4Neuronal Pas4Class E Basic Helix-Loop-Helix Protein 79Bhlhe79Hlh-Pas Transcription Factor NxfPas Domain-Containing Protein 10 |
| Database Links | Reactome: R-HSA-9768778Reactome: R-HSA-9768919 |
| Cellular Localisation | Nucleus |
| Alternative Peptide Names | Neuronal Pas Domain-Containing Protein 4 proteinNeuronal Pas4 proteinClass E Basic Helix-Loop-Helix Protein 79 proteinBhlhe79 proteinHlh-Pas Transcription Factor Nxf proteinPas Domain-Containing Protein 10 proteinNPAS4 proteinBHLHE79 proteinNXF proteinPASD10 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
