| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
NEDD4 Positive Control for STJ506276 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:250 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
Nedd4 |
| Gene ID: |
25489 |
| Uniprot ID: |
NEDD4_RAT |
| Specificity: |
This is positive control is recommended for use in combination with NEDD4 antibody STJ506276. |
| Tissue Specificity | Ubiquitously expressed. Expression is highest in lung, kidney and brain. |
| Post Translational Modifications | Undergoes 'Lys-29'-linked auto-ubiquitination at Lys-847 and serves as a scaffold for recruiting USP13 to form an NEDD4-USP13 deubiquitination complex. |
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Specifically ubiquitinates 'Lys-63' in target proteins. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Promotes ubiquitination of RAPGEF2. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Is involved in ubiquitination of ERBB4 intracellular domain E4ICD. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1. Ubiquitinates DAZAP2, leading to its proteasomal degradation. Ubiquitinates POLR2A. Functions as a platform to recruit USP13 to form an NEDD4-USP13 deubiquitination complex that plays a critical role in cleaving the 'Lys-48'-linked ubiquitin chains of VPS34 and then stabilizing VPS34, thus promoting the formation of autophagosomes. |
| Peptide Name | E3 Ubiquitin-Protein Ligase Nedd4Hect-Type E3 Ubiquitin Transferase Nedd4 |
| Database Links | Reactome: R-RNO-1169408Reactome: -RNO-1253288Reactome: -RNO-8948747Reactome: -RNO-8948751Reactome: -RNO-983168 |
| Cellular Localisation | CytoplasmNucleusCell MembranePeripheral Membrane ProteinPredominantly Cytoplasmic But Also Located In The NucleusRecruited To The Plasma Membrane By Grb10Once Complexed With Grb10 And Igf1rFollows Igf1r InternalizationRemaining Associated With Early EndosomesUncouples From Igf1r-Containing Endosomes Before The Sorting Of The Receptor To The Lysosomal CompartmentMay Be Recruited To Exosomes By Ndfip1 |
| Alternative Peptide Names | E3 Ubiquitin-Protein Ligase Nedd4 proteinHect-Type E3 Ubiquitin Transferase Nedd4 proteinNedd4 proteinNedd4a proteinRpf1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
