Mouse VEGFR1/Flt-1 protein (Recombinant) (hIgG-Tag) (STJP019568)
SPECIFICATIONS
HostHEK293 Cells
ImmunogenMouse
General Information
| Short Description | Recombinant-Mouse VEGFR1/Flt-1-hIgG-Tag protein was developed from hek293 cells and has a target region of hIgG-Tag. For use in research applications. |
| Applications | SDS-PAGE/Bioactivity |
| Host | HEK293 Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
| Immunoreactivity | Measured by its ability to inhibit proliferation using HUVEC human umbilical vein endothelial cells in the presence of Human VEGF165. The ED50 range ≤ 50ng/ml. |
Target Information
| Gene Symbol | Flt1 |
| Gene ID | 14254 |
| Uniprot ID | VGFR1_MOUSE |
| Accession Number | NP_034358.2 |
| Immunogen | Mouse |
| Immunogen Region | 23-759aa |
| Immunogen Sequence | YGSGSKLKVP ELSLKGTQHV MQAGQTLFLK CRGEAAHSWS LPTTVSQEDK RLSITPPSAC GRDNRQFCST LTLDTAQANH TGLYTCRYLP TSTSKKKKAE SSIYIFVSDA GSPFIEMHTD IPKLVHMTEG RQLIIPCRVT SPNVTVTLKK FPFDTLTPDG QRITWDSRRG FIIANATYKE IGLLNCEATV NGHLYQTNYL THRQTNTILD VQIRPPSPVR LLHGQTLV |
Additional Info
| Post Translational Modifications | N-glycosylated. Ubiquitinated after VEGFA-mediated autophosphorylation, leading to proteolytic degradation. Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-1169 is important for interaction with PLCG. Phosphorylation at Tyr-1213 is important for interaction with PIK3R1, PTPN11, GRB2, and PLCG. Phosphorylation at Tyr-1328 is important for endocytosis and for interaction with CBL, NCK1 and CRK. Is probably dephosphorylated by PTPRB. |
| Function | Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Acts as a positive regulator of postnatal retinal hyaloid vessel regression. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, and proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts. Has very high affinity for VEGFA and relatively low protein kinase activity.may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to the activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC, YES1 and PLCG, and may also phosphorylate CBL. Promotes phosphorylation of AKT1 and PTK2/FAK1. |
| Protein Name | Vascular Endothelial Growth Factor Receptor 1Vegfr-1Embryonic Receptor Kinase 2Fms-Like Tyrosine Kinase 1Flt-1Tyrosine-Protein Kinase Receptor Flt |
| Database Links | Reactome: R-MMU-194306Reactome: -MMU-195399 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinEndosomeAutophosphorylation Promotes Ubiquitination And Endocytosis |
| Alternative Protein Names | Vascular Endothelial Growth Factor Receptor 1 proteinVegfr-1 proteinEmbryonic Receptor Kinase 2 proteinFms-Like Tyrosine Kinase 1 proteinFlt-1 proteinTyrosine-Protein Kinase Receptor Flt proteinFlt1 proteinEmrk2 proteinFlt proteinVegfr1 protein |
Information sourced from Uniprot.org