| Host: |
HEK293 cells |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Mouse VEGFR-2/KDR/CD309-C-His protein was developed from hek293 cells and has a target region of C-His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Immunoreactivity: |
Measured by its ability to inhibit the VEGF-dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 6-23 pg/mL in the presence of 5 ng/mL recombinant mouse VEGF. |
| Gene Symbol: |
Kdr |
| Gene ID: |
16542 |
| Uniprot ID: |
VGFR2_MOUSE |
| Immunogen: |
Recombinant Mouse VEGFR-2/KDR/CD309 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala20-Glu762) of mouse VEGFR2/KDR (Accession #NP_034742.2) fused with a 6×His tag at the C-terminus. |
| Post Translational Modifications | N-glycosylated. Ubiquitinated. Tyrosine phosphorylation of the receptor promotes its poly-ubiquitination, leading to its degradation via the proteasome or lysosomal proteases. Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-949 is important for interaction with SH2D2A/TSAD and VEGFA-mediated reorganization of the actin cytoskeleton. Phosphorylation at Tyr-1173 is important for interaction with PLCG1 and SHB. Phosphorylation at Tyr-1212 is important for interaction with NCK1 and FYN. Dephosphorylated by PTPRJ at Tyr-799, Tyr-949, Tyr-994, Tyr-1052, Tyr-1057, Tyr-1173 and Tyr-1212. The inhibitory disulfide bond between Cys-1022 and Cys-1043 may serve as a specific molecular switch for H(2)S-induced modification that regulates KDR/VEGFR2 function. |
| Function | Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as a negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and by preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC. |
| Protein Name | Vascular Endothelial Growth Factor Receptor 2Vegfr-2Fetal Liver Kinase 1Flk-1Kinase NykProtein-Tyrosine Kinase Receptor Flk-1Cd Antigen Cd309 |
| Database Links | Reactome: R-MMU-194306Reactome: -MMU-195399Reactome: -MMU-4420097Reactome: -MMU-5218921 |
| Cellular Localisation | Cell JunctionEndoplasmic ReticulumCell MembraneColocalizes With Ern1 And Xbp1 In The Endoplasmic Reticulum In Endothelial Cells In A Vascular Endothelial Growth Factor (Vegf)-Dependent MannerLocalized With Rap1a At Cell-Cell JunctionsIsoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinCytoplasmNucleusCytoplasmic VesicleEarly EndosomeDetected On Caveolae-Enriched Lipid Rafts At The Cell SurfaceIs Recycled From The Plasma Membrane To Endosomes And Back AgainPhosphorylation Triggered By Vegfa Binding Promotes Internalization And Subsequent DegradationVegfa Binding Triggers Internalization And Translocation To The NucleusIsoform 2: Secreted |
| Alternative Protein Names | Vascular Endothelial Growth Factor Receptor 2 proteinVegfr-2 proteinFetal Liver Kinase 1 proteinFlk-1 proteinKinase Nyk proteinProtein-Tyrosine Kinase Receptor Flk-1 proteinCd Antigen Cd309 proteinKdr proteinFlk-1 proteinFlk1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
