Mouse VEGFA (VEGFA) Antibody Pair (STJAP00749)
General Information
| Short Description | This VEGFA antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R09-1U1/R01-1L3 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | Vegfa |
| Gene ID | 22339 |
| Uniprot ID | VEGFA_MOUSE |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | In developing embryos, expressed mainly in the choroid plexus, paraventricular neuroepithelium, placenta and kidney glomeruli. Also found in bronchial epithelium, adrenal gland and in seminiferous tubules of testis. High expression continues in kidney glomeruli and choroid plexus in adults. |
| Post Translational Modifications | Vascular endothelial growth factor A, long form: Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form. |
| Function | N-VEGF: Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A. Involved in protecting cells from hypoxia-mediated cell death. VEGFA: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Also binds the DEAR/FBXW7-AS1 receptor. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis. |
| Protein Name | Vascular Endothelial Growth Factor A - Long FormL-VegfVascular Permeability FactorVpf Cleaved Into - N-Vegf - Vegfa |
| Database Links | Reactome: R-MMU-114608Reactome: -MMU-194313Reactome: -MMU-195399Reactome: -MMU-4420097Reactome: -MMU-5218921 |
| Cellular Localisation | N-Vegf: CytoplasmNucleusCytoplasmic In Normoxic Conditions And Localizes To The Nucleus Under Hypoxic ConditionsVegfa: SecretedIsoform L-Vegf-2: Endoplasmic ReticulumGolgi ApparatusSecretedExtracellular SpaceExtracellular MatrixIsoform Vegf-1: SecretedIsoform Vegf-2: SecretedIsoform Vegf-3: Cell MembranePeripheral Membrane ProteinRemains Cell-Surface Associated Unless Released By Heparin |
| Alternative Antibody Pair Names | Vascular Endothelial Growth Factor A - Long Form antibody pairL-Vegf antibody pairVascular Permeability Factor antibody pairVpf Cleaved Into - N-Vegf - Vegfa antibody pairVegfa antibody pairVegf antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse VEGFA (VEGFA) Antibody Pair (STJAP00749)
This antibody pair is designed to detect mouse VEGFA (UniProt Q00731) and has confirmed reactivity against mouse samples only.
This antibody pair has been validated for use in ELISA. It is intended for research use only and has not been validated for diagnostic or therapeutic applications.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated at 1 mg/mL, formulated in 0.01M PBS at pH 7.2, and purified by affinity chromatography. Clone IDs are R09-1U1 and R01-1L3 for the capture and detection antibodies, respectively.
Optimal working concentrations for both antibodies are to be determined by the end user through titration, as no fixed dilution range is specified for this product.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C, and avoid repeated freeze/thaw cycles.
Both antibodies in this pair were raised in rabbit using a recombinant mouse VEGFA protein as the immunogen.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.