Mouse VEGF-A/VEGF164 protein (Recombinant) (N-His) (STJP001145)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Mouse VEGF-A/VEGF164 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ala27-Arg190) of mouse VEGF 164 (Accession #NP_001273986.1.) fused with a 6×His tag at the N-terminus.
General Information
| Short Description | Recombinant-Mouse VEGF-A/VEGF164-N-His protein was developed in hek293 cells using the region Ala27-Arg190. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse VEGF164 at 1 Mu g/mL (100 Mu L/well) can bind Recombinant Human VEGFR2 with a linear range of 8-30 ng/mL.|2.Measured in a cell proliferation assay using human umbi |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | Vegfa |
| Gene ID | 22339 |
| Uniprot ID | VEGFA_MOUSE |
| Immunogen | Recombinant Mouse VEGF-A/VEGF164 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ala27-Arg190) of mouse VEGF 164 (Accession #NP_001273986.1.) fused with a 6×His tag at the N-terminus. |
| Immunogen Region | Ala27-Arg190 |
| Immunogen Sequence | APTTEGEQKSHEVIKFMDVY QRSYCRPIETLVDIFQEYPD EIEYIFKPSCVPLMRCAGCC NDEALECVPTSESNITMQIM RIKPHQSQHIGEMSFLQHSR CECRPKKDRTKPENHCEPCS ERRKHLFVQDPQTCKCSCKN TDSRCKARQLELNERTCRCD KPRR |
Additional Info
| Post Translational Modifications | Vascular endothelial growth factor A, long form: Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form. |
| Function | N-VEGF: Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A. Involved in protecting cells from hypoxia-mediated cell death. VEGFA: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Also binds the DEAR/FBXW7-AS1 receptor. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis. |
| Protein Name | Vascular Endothelial Growth Factor A - Long FormL-VegfVascular Permeability FactorVpf Cleaved Into - N-Vegf - Vegfa |
| Database Links | Reactome: R-MMU-114608Reactome: -MMU-194313Reactome: -MMU-195399Reactome: -MMU-4420097Reactome: -MMU-5218921 |
| Cellular Localisation | N-Vegf: CytoplasmNucleusCytoplasmic In Normoxic Conditions And Localizes To The Nucleus Under Hypoxic ConditionsVegfa: SecretedIsoform L-Vegf-2: Endoplasmic ReticulumGolgi ApparatusSecretedExtracellular SpaceExtracellular MatrixIsoform Vegf-1: SecretedIsoform Vegf-2: SecretedIsoform Vegf-3: Cell MembranePeripheral Membrane ProteinRemains Cell-Surface Associated Unless Released By Heparin |
| Alternative Protein Names | Vascular Endothelial Growth Factor A - Long Form proteinL-Vegf proteinVascular Permeability Factor proteinVpf Cleaved Into - N-Vegf - Vegfa proteinVegfa proteinVegf protein |
Information sourced from Uniprot.org