Host: | HEK293 cells |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
Immunoreactivity: | Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to mouse VCAM1 coated plates (10 Mu g/mL with 100 Mu L/well) , approximately 80%-90% cells will ad |
Immunogen: | Recombinant Mouse VCAM-1/CD106 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Glu698) of mouse VCAM-1/CD106 (Accession #NP_035823.3) fused with a Fc, 6×His tag at the C-terminus. |
Immunogen Sequence: | MPVKMVAVLGASTVLWILFA VSQAFKIEISPEYKTIAQIG DSMALTCSTTGCESPLFSWR TQIDSPLNAKVRTEGSKSVL TMEPVSFENEHSYLCTATCG SGKLERSIHVDIYSFPKDPE IQFSGPLEVGKPVTVKCLAP DIYPVYRLEIDLFKGDQLMN RQEFSSEEMTKSLETKSLEV TFTPVIEDIGKALVCRAKLH IDQIDSTLKERETVKELQVY ISPRNTTISVHPSTRLQEG |