Mouse RSPO1 protein (Recombinant-Active) (C-His) (STJP012334)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenMus musculus (Mouse)
General Information
| Short Description | Recombinant-Active-Mouse RSPO1-C-His protein was developed from e.coli and has a target region of C-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | <1 EU/Mu g of the protein by the LAL method. |
| Immunoreactivity | Measured by its ability to induce Topflash reporter activity in human embryonic kidney cells (HEK293T). The typical ED50 is 50-200 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a. |
Target Information
| Gene Symbol | Rspo1 |
| Gene ID | 192199 |
| Uniprot ID | RSPO1_MOUSE |
| Immunogen | Mus musculus (Mouse) |
| Immunogen Region | Ser21-Gly209 |
Additional Info
| Post Translational Modifications | C-, and N-glycosylated. N-glycosylation at Asn-137, negatively influences its secretion and enhancing effect on Wnt/beta-catenin signaling. C-mannosylation at Trp-156 by DPY19L3 is required for its secretion an regulates the enhancing activity of Wnt signaling. |
| Function | Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Regulates Wnt signaling by antagonizing DKK1/KREM1-mediated internalization of LRP6 through an interaction with KREM1. |
| Protein Name | R-Spondin-1Cysteine-Rich And Single Thrombospondin Domain-Containing Protein 3Cristin-3Mcristin-3Roof Plate-Specific Spondin-1 |
| Database Links | Reactome: R-MMU-4641263 |
| Cellular Localisation | SecretedNucleusSeems To Mainly Localize To Nucleoli |
| Alternative Protein Names | R-Spondin-1 proteinCysteine-Rich And Single Thrombospondin Domain-Containing Protein 3 proteinCristin-3 proteinMcristin-3 proteinRoof Plate-Specific Spondin-1 proteinRspo1 protein |
Information sourced from Uniprot.org