| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.113ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This RAD50 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse tissue homogenates, cell lysates or other biological fluids.. |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Rad50 |
| Uniprot ID: |
RAD50_MOUSE |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | In adult, it is expressed at very low level in most tissues, except in heart, lung and aorta. Expressed at high level in testis. |
| Post Translational Modifications | Phosphorylation at Ser-635 by ATM in response to DNA damage is required for double-strand break (DSB) repair. |
| Function | Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. The MRN complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11, to initiate end resection, which is required for single-strand invasion and recombination. Within the complex, RAD50 is both required to bind DNA ends and hold them in close proximity and regulate the activity of MRE11. RAD50 provides an ATP-dependent control of MRE11 by positioning DNA ends into the MRE11 active site: ATP-binding induces a large structural change from an open form with accessible MRE11 nuclease sites into a closed form. The MRN complex is also required for DNA damage signaling via activation of the ATM and ATR kinases: the nuclease activity of MRE11 is not required to activate ATM and ATR. The MRN complex is also required for the processing of R-loops. In telomeres the MRN complex may modulate t-loop formation. |
| Protein Name | Dna Repair Protein Rad50Mrad50 |
| Database Links | |
| Cellular Localisation | NucleusChromosomeTelomereLocalizes To Discrete Nuclear Foci After Treatment With Genotoxic AgentsLocalizes To Dna Double-Strand Breaks (Dsbs) |
| Alternative ELISA Names | Dna Repair Protein Rad50 ELISA kitMrad50 ELISA kitRad50 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
