| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 46.88pg/mL |
| Detection Limit: | 78.13-5000pg/mL |
| Short Description : | This Pro-MMP-9 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Mmp9 |
| Gene ID: | 17395 |
| Uniprot ID: | MMP9_MOUSE |
| Specificity: | This kit recognizes Mouse Pro-MMP-9 in samples. No significant cross-reactivity or interference between Mouse ProMMP-9 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. |
| Protein Name | Matrix Metalloproteinase-9 Mmp-9 92 Kda Gelatinase 92 Kda Type Iv Collagenase Gelatinase B Gelb |
| Database Links | Reactome: R-MMU-1433557 Reactome: -MMU-1442490 Reactome: -MMU-1474228 Reactome: -MMU-1592389 Reactome: -MMU-2022090 Reactome: -MMU-3928665 Reactome: -MMU-6798695 Reactome: -MMU-9009391 |
| Cellular Localisation | Secreted Extracellular Space Extracellular Matrix |
| Alternative ELISA Names | Matrix Metalloproteinase-9 ELISA kit Mmp-9 ELISA kit 92 Kda Gelatinase ELISA kit 92 Kda Type Iv Collagenase ELISA kit Gelatinase B ELISA kit Gelb ELISA kit Mmp9 ELISA kit Clg4b ELISA kit |
| Specificity | This kit recognizes Mouse Pro-MMP-9 in samples. No significant cross-reactivity or interference between Mouse Pro-MMP-9 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 85-100 | 91 |
| EDTA plasma (n=8) | 91-105 | 96 |
| Cell culture media (n=8) | 89-101 | 95 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 253.11 | 435.12 | 1790.24 | 258.34 | 407.40 | 1655.43 |
| Standard deviation | 17.26 | 22.54 | 88.08 | 16.82 | 17.88 | 69.53 |
| CV (%) | 6.82 | 5.18 | 4.92 | 6.51 | 4.39 | 4.20 |