| Short Description : | Recombinant-Mouse Osteopontin/SPP1-C-His protein was developed in hek293 cells using the region Leu17-Asn294. For use in research applications. |
| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: | Measured by the ability of the immobilized protein to support the adhesion of B16 cells.When 5×104 cells/well are added to a Recombinant Mouse (rm) Osteopontin/OPN coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 |
| Determination Method: | <1 EU/Mu g |
| Gene Symbol: | Spp1 |
| Gene ID: | 20750 |
| Uniprot ID: | OSTP_MOUSE |
| Immunogen: | Recombinant Mouse Osteopontin/SPP1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu17-Asn294) of mouse Osteopontin/SPP1 (Accession #NP_033289.2) fused with a 6×His tag at the C-terminus. |
| Immunogen Region: | Leu17-Asn294 |
| Immunogen Sequence: | LPVKVTDSGSSEEKLYSLHP DPIATWLVPDPSQKQNLLAP QNAVSSEEKDDFKQETLPSN SNESHDHMDDDDDDDDDDGD HAESEDSVDSDESDESHHSD ESDETVTASTQADTFTPIVP TVDVPNGRGDSLAYGLRSKS RSFQVSDEQYPDATDEDLTS HMKSGESKESLDVIPVAQLL SMPSDQDNNGKGSHESSQLD EPSLETHRLEHSKESQESAD QSDVIDSQASSKASLEHQS |
| Function | Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. |
| Protein Name | Osteopontin2arBone Sialoprotein 1Calcium Oxalate Crystal Growth Inhibitor ProteinEarly T-Lymphocyte Activation 1 ProteinMinopontinSecreted Phosphoprotein 1Spp-1 |
| Database Links | Reactome: R-MMU-1474228Reactome: -MMU-186797Reactome: -MMU-216083Reactome: -MMU-381426Reactome: -MMU-8957275 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Osteopontin protein2ar proteinBone Sialoprotein 1 proteinCalcium Oxalate Crystal Growth Inhibitor Protein proteinEarly T-Lymphocyte Activation 1 Protein proteinMinopontin proteinSecreted Phosphoprotein 1 proteinSpp-1 proteinSpp1 proteinEta-1 proteinOp proteinSpp-1 protein |
Information sourced from Uniprot.org
