Mouse Nestin (Nestin) Antibody Pair (STJAP00798)
General Information
| Short Description | This Nestin antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R65-5M-1/R98-7L-2 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | Nes |
| Gene ID | 18008 |
| Uniprot ID | NEST_MOUSE |
| Immunogen | Recombinant protein |
Additional Info
| Post Translational Modifications | Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. |
| Function | Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. |
| Protein Name | Nestin |
| Database Links | |
| Cellular Localisation | |
| Alternative Antibody Pair Names | Nestin antibody pairNes antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse Nestin (Nestin) Antibody Pair (STJAP00798)
This antibody pair is designed to detect mouse Nestin (UniProt Q6P5H2) and has confirmed reactivity against the mouse target only. It is intended for use in sandwich ELISA-based research applications.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated at 1 mg/mL in 0.01M PBS, pH 7.2. Clone IDs are R65-5M-1 and R98-7L-2, and both have been affinity purified.
This antibody pair has been validated for ELISA. No other assay formats are documented for this product.
Both antibodies were raised in rabbit against a recombinant protein immunogen corresponding to mouse Nestin. No specific immunogen region is documented for this product.
Working concentrations for both the capture and detection antibodies are to be determined by the end user and should be optimized empirically for the specific experimental conditions and sample types being used.
Both components should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C. Repeated freeze/thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.