| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | <0.1 EU/Mu g |
| Immunoreactivity: | Measured by the ability of the immobilized protein to support the adhesion of NIH 3T3 mouse embryonic fibroblast cells. When 5 x 104 cells/well are added to recombinant mouse Nectin-4 coated plates (10 Mu g/mL with 100 Mu L/well) , approximately 30%- |
| Gene Symbol: | Nectin4 |
| Gene ID: | 71740 |
| Uniprot ID: | NECT4_MOUSE |
| Immunogen Region: | Gly31-Ser347 |
| Immunogen: | Recombinant Mouse Nectin-4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly31-Ser347) of mouse Nectin-4 (Accession #NP_082169.2) fused with a 6ÃHis tag at the C-terminus. |
| Immunogen Sequence: | GELETSDVVTVVLGQDAKLP CFYRGDPDEQVGQVAWARVD PNEGIRELALLHSKYGLHVN PAYEDRVEQPPPPRDPLDGS VLLRNAVQADEGEYECRVST FPAGSFQARMRLRVLVPPLP SLNPGPPLEEGQGLTLAASC TAEGSPAPSVTWDTEVKGTQ SSRSFTHPRSAAVTSEFHLV PSRSMNGQPLTCVVSHPGLL QDRRITHTLQVAFLAEASVR GLEDQNLWQVGREGATLKC |
| Function | Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Plays a role in the senescence-associated cell size enlargement via SFK/PI3K/Rac1 and thus promotes senescent cell survival. Also participates in the innate immune response by acting as a ligand for the receptor TIGIT to inhibit NK-cell activity. |
| Protein Name | Nectin-4Ig Superfamily Receptor LnirNectin Cell Adhesion Molecule 4Poliovirus Receptor-Related Protein 4 |
| Database Links | Reactome: R-MMU-418990Reactome: -MMU-420597 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell JunctionAdherens JunctionColocalizes With Afdn At Cadherin-Based Adherens Junctions |
| Alternative Protein Names | Nectin-4 proteinIg Superfamily Receptor Lnir proteinNectin Cell Adhesion Molecule 4 proteinPoliovirus Receptor-Related Protein 4 proteinNectin4 proteinLnir proteinPrr4 proteinPvrl4 protein |
Information sourced from Uniprot.org
