Function | Can degrade fibrillar type I, II, and III collagens. May play a role in the degradation of collagen fibers during uterine involution. |
Protein Name | Neutrophil CollagenaseCollagenase 2Matrix Metalloproteinase-8Mmp-8 |
Database Links | Reactome: R-MMU-1442490Reactome: -MMU-1474228Reactome: -MMU-1592389Reactome: -MMU-6798695 |
Cellular Localisation | Cytoplasmic GranuleSecretedExtracellular SpaceExtracellular MatrixStored In Intracellular Granules And Released During Inflammatory Conditions |
Alternative ELISA Names | Neutrophil Collagenase ELISA kitCollagenase 2 ELISA kitMatrix Metalloproteinase-8 ELISA kitMmp-8 ELISA kitMmp8 ELISA kit |
Specificity | This kit recognizes Mouse MMP-8 in samples. No significant cross-reactivity or interference between Mouse MMP-8 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 93-110 | 101 |
EDTA plasma (n=8) | 96-110 | 102 |
Cell culture media (n=8) | 85-98 | 92 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (ng/mL) | 0.53 | 0.98 | 4.34 | 0.56 | 0.90 | 4.58 |
Standard deviation | 0.03 | 0.05 | 0.14 | 0.04 | 0.05 | 0.16 |
CV (%) | 5.65 | 5.23 | 3.25 | 6.26 | 5.41 | 3.60 |