Mouse LYPLA1 protein (Recombinant) (His-Tag) (STJP016860)
SPECIFICATIONS
HostE.coli
Immunogenmouse
General Information
| Short Description | Recombinant-Mouse LYPLA1-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol, 0.1M NaCl, 1mM DTT |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Lypla1 |
| Gene ID | 18777 |
| Uniprot ID | LYPA1_MOUSE |
| Accession Number | NP_032892 |
| Immunogen | mouse |
| Immunogen Region | 1-230aa |
| Immunogen Sequence | MGSSHHHHHH SSGLVPRGSH MCGNNMSAPM PAVVPAARKA TAAVIFLHGL GDTGHGWAEA FAGIKSPHIK YICPHAPVMP VTLNMNMAMP SWFDIVGLSP DSQEDESGIK QAAETVKALI DQEVKNGIPS NRIILGGFSQ GGALSLYTAL TTQQKLAGVT ALSCWLPLRA SFSQGPINSA NRDISVLQCH GDCDPLVPLM FGSLTVERLK ALINPANVTF KIYEGMMH |
Additional Info
| Function | Acts as an acyl-protein thioesterase. Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Acts as a palmitoyl thioesterase that catalyzes depalmitoylation of proteins, such as ADRB2, KCNMA1 and SQSTM1. Acts as a negative regulator of autophagy by mediating palmitoylation of SQSTM1, decreasing affinity between SQSTM1 and ATG8 proteins and recruitment of ubiquitinated cargo proteins to autophagosomes. Acts as a lysophospholipase and hydrolyzes lysophosphatidylcholine (lyso-PC). Also hydrolyzes lysophosphatidylethanolamine (lyso-PE), lysophosphatidylinositol (lyso-PI) and lysophosphatidylserine (lyso-PS). Has much higher thioesterase activity than lysophospholipase activity. Contributes to the production of lysophosphatidic acid (LPA) during blood coagulation by recognizing and cleaving plasma phospholipids to generate lysophospholipids which in turn act as substrates for ENPP2 to produce LPA. |
| Protein Name | Acyl-Protein Thioesterase 1Apt-1Lysophospholipase 1Lysophospholipase ILpl-ILysopla IPalmitoyl-Protein Hydrolase |
| Database Links | Reactome: R-MMU-203615Reactome: -MMU-9648002 |
| Cellular Localisation | CytoplasmCell MembraneNucleus MembraneEndoplasmic ReticulumShows Predominantly A Cytoplasmic Localization With A Weak Expression In The Cell MembraneNuclear Membrane And Endoplasmic Reticulum |
| Alternative Protein Names | Acyl-Protein Thioesterase 1 proteinApt-1 proteinLysophospholipase 1 proteinLysophospholipase I proteinLpl-I proteinLysopla I proteinPalmitoyl-Protein Hydrolase proteinLypla1 proteinApt1 proteinPla1a protein |
Information sourced from Uniprot.org