| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.108ng/mL |
| Detection Limit: | 0.312-20ng/mL |
| Short Description : | This LPCAT2 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse tissue homogenates or other biological fluids.. |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | Lpcat2 |
| Gene ID: | 270084 |
| Uniprot ID: | PCAT2_MOUSE |
| Sample Type: | tissue homogenates or other biological fluids. |
| Tissue Specificity | Highest expression is found in resident macrophages and casein-induced neutrophils followed by skin, colon, spleen and thioglycollate-induced macrophages. Detected in erythroleukemic cells but not in reticulocytes. |
| Post Translational Modifications | |
| Function | Exhibits both acyltransferase and acetyltransferase activities. Activity is calcium-dependent. Catalyzes the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC). Catalyzes the conversion 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Involved in platelet-activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF). Also converts lyso-PAF to 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine (PC), a major component of cell membranes and a PAF precursor. Under resting conditions, acyltransferase activity is preferred. Upon acute inflammatory stimulus, acetyltransferase activity is enhanced and PAF synthesis increases. Involved in the regulation of lipid droplet number and size. |
| Protein Name | Lysophosphatidylcholine Acyltransferase 2 Lpc Acyltransferase 2 Lpcat-2 Lysopc Acyltransferase 2 1-Acylglycerol-3-Phosphate O-Acyltransferase 11 1-Agp Acyltransferase 11 1-Agpat 11 1-Acylglycerophosphocholine O-Acyltransferase 1-Alkenylglycerophosphocholine O-Acyltransferase 1-Alkylglycerophosphocholine O-Acetyltransferase Acetyl-Coa -Lyso-Platelet-Activating Factor Acetyltransferase Acetyl-Coa -Lyso-Paf Acetyltransferase Lyso-Paf Acetyltransferase Lysopafat Acyltransferase-Like 1 |
| Database Links | Reactome: R-MMU-1482788 |
| Cellular Localisation | Endoplasmic Reticulum Membrane Single-Pass Type Ii Membrane Protein Golgi Apparatus Membrane Cell Membrane Lipid Droplet |
| Alternative ELISA Names | Lysophosphatidylcholine Acyltransferase 2 ELISA kit Lpc Acyltransferase 2 ELISA kit Lpcat-2 ELISA kit Lysopc Acyltransferase 2 ELISA kit 1-Acylglycerol-3-Phosphate O-Acyltransferase 11 ELISA kit 1-Agp Acyltransferase 11 ELISA kit 1-Agpat 11 ELISA kit 1-Acylglycerophosphocholine O-Acyltransferase ELISA kit 1-Alkenylglycerophosphocholine O-Acyltransferase ELISA kit 1-Alkylglycerophosphocholine O-Acetyltransferase ELISA kit Acetyl-Coa -Lyso-Platelet-Activating Factor Acetyltransferase ELISA kit Acetyl-Coa -Lyso-Paf Acetyltransferase ELISA kit Lyso-Paf Acetyltransferase ELISA kit Lysopafat ELISA kit Acyltransferase-Like 1 ELISA kit Lpcat2 ELISA kit Aytl1 ELISA kit Aytl1a ELISA kit Lpcat2a ELISA kit |
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Information sourced from Uniprot.org