Protein Name | Interleukin-4Il-4B-Cell Igg Differentiation FactorB-Cell Growth Factor 1B-Cell Stimulatory Factor 1Bsf-1Igg1 Induction FactorLymphocyte Stimulatory Factor 1 |
Database Links | Reactome: R-MMU-6785807 |
Cellular Localisation | Secreted |
Alternative ELISA Names | Interleukin-4 ELISA kitIl-4 ELISA kitB-Cell Igg Differentiation Factor ELISA kitB-Cell Growth Factor 1 ELISA kitB-Cell Stimulatory Factor 1 ELISA kitBsf-1 ELISA kitIgg1 Induction Factor ELISA kitLymphocyte Stimulatory Factor 1 ELISA kitIl4 ELISA kitIl-4 ELISA kit |
Specificity | This kit recognizes Mouse IL-4 in samples. No significant cross-reactivity or interference between Mouse IL-4 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 86-99 | 91 |
EDTA plasma (n=8) | 95-111 | 101 |
Cell culture media (n=8) | 92-108 | 98 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 97.60 | 252.20 | 947.10 | 99.90 | 274.50 | 966.50 |
Standard deviation | 6.50 | 11.30 | 30.30 | 6.40 | 14.00 | 36.70 |
CV (%) | 6.66 | 4.48 | 3.20 | 6.41 | 5.10 | 3.80 |