| Protein Name | Interleukin-12 Subunit BetaIl-12bCytotoxic Lymphocyte Maturation Factor 40 Kda SubunitClmf P40Il-12 Subunit P40 |
| Database Links | Reactome: R-MMU-9020591Reactome: -MMU-9020933 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Interleukin-12 Subunit Beta ELISA kitIl-12b ELISA kitCytotoxic Lymphocyte Maturation Factor 40 Kda Subunit ELISA kitClmf P40 ELISA kitIl-12 Subunit P40 ELISA kitIl12b ELISA kit |
| Specificity | This kit recognizes Mouse IL-12 in samples. No significant cross-reactivity or interference between Mouse IL-12 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 84-96 | 90 |
| EDTA plasma (n=8) | 88-100 | 95 |
| Cell culture media (n=8) | 95-110 | 102 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 0.94 | 2.48 | 6.82 | 0.91 | 2.37 | 6.40 |
| Standard deviation | 0.06 | 0.13 | 0.35 | 0.05 | 0.12 | 0.33 |
| CV (%) | 6.48 | 5.39 | 5.08 | 5.61 | 4.97 | 5.19 |
