Mouse IL-36 gamma/IL-1F9 protein (Recombinant) (N-His) (STJP002967)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenRecombinant Mouse IL-36 gamma/IL-1F9 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly13-Ser164) of mouse IL36G (Accession #) fused with and a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Mouse IL-36 gamma/IL-1F9-N-His protein was developed in e.coli using the region Gly13-Ser164. For use in research applications. |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677. The ED 50 for this effect is 0.1-0.4 Mu g/mL, corresponding to a specific activity of 2.5×10 |
| Determination Method | < 0.01 EU/Mu g |
Target Information
| Gene Symbol | Il36gUniprot ID=IL36G_MOUSE" |
| Immunogen | Recombinant Mouse IL-36 gamma/IL-1F9 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly13-Ser164) of mouse IL36G (Accession #) fused with and a 6×His tag at the C-terminus. |
| Immunogen Region | Gly13-Ser164 |
| Immunogen Sequence | MQIFVKTLTGKTITLEVEPS DTIENVKAKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGG |
Additional Info
| Post Translational Modifications | N-terminal truncation leads to a dramatic enhancement of its activity (>1000-fold). Proteolytically cleaved by cathepsin CTSG. |
| Function | Functions as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2/IL1RL2. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response.similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. May play a role in pro-inflammatory responses during particular neutrophilic airway inflammation. May be involved in the innate immune response to fungal pathogens. Induces the production of pro-inflammatory cytokines in bone marrow-derived dendritic cells (BMDCs), including IL-12, Il-1 beta, IL-6, TNF-alpha and IL-23. Involved in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II. Induces the production of IFN-gamma, IL-4 and IL-17 by cultured CD4(+) T-cells and splenocytes. |
| Protein Name | Interleukin-36 GammaInterleukin-1 Family Member 9Il-1f9 |
| Cellular Localisation | CytoplasmSecretedThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion |
| Alternative Protein Names | Interleukin-36 Gamma proteinInterleukin-1 Family Member 9 proteinIl-1f9 proteinIl36g proteinIl1f9 protein |
Information sourced from Uniprot.org