| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 5.88 pg/mL |
| Detection Limit: | 12.5-800 pg/mL |
| Short Description : | This IL-18 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Il18 |
| Gene ID: | 16173 |
| Uniprot ID: | IL18_MOUSE |
| Specificity: | This kit recognizes Mouse IL-18 in samples.No significant cross-reactivity or interference between Mouse IL-18 and analogues was observed |
| Sample Type: | serum, plasma and other biological fluids |
| Post Translational Modifications | The pro-IL-18 precursor is processed by CASP1 to yield its mature, active form. The pro-IL-18 precursor is however not processed by Casp4/Casp11 in rodents. The pro-IL-18 precursor features autoinhibitory interactions between the propeptide and the post-cleavage-site region, preventing recognition by the IL18R1 receptor. Processing by CASP1 induces conformational changes to generate critical receptor-binding sites. The mature form is then secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. In contrast, cleavage by CASP3 inactivates IL18. |
| Function | Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. |
| Protein Name | Interleukin-18 Il-18 Interferon Gamma-Inducing Factor Ifn-Gamma-Inducing Factor Interleukin-1 Gamma Il-1 Gamma |
| Database Links | Reactome: R-MMU-448706 Reactome: -MMU-5620971 Reactome: -MMU-9012546 |
| Cellular Localisation | Cytoplasm Secreted The Precursor Is Cytosolic In Response To Inflammasome-Activating Signals Cleaved And Secreted Mature Form Is Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) Pore In Contrast The Precursor Form Is Not Released Due To The Presence Of An Acidic Region That Is Proteolytically Removed By Casp1 During Maturation The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10 |
| Alternative ELISA Names | Interleukin-18 ELISA kit Il-18 ELISA kit Interferon Gamma-Inducing Factor ELISA kit Ifn-Gamma-Inducing Factor ELISA kit Interleukin-1 Gamma ELISA kit Il-1 Gamma ELISA kit Il18 ELISA kit Igif ELISA kit |
| Specificity | This kit recognizes Mouse IL-18 in samples. No significant cross-reactivity or interference between Mouse IL-18 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 84-99 | 90 |
| EDTA plasma (n=8) | 94-107 | 100 |
| Cell culture media (n=8) | 88-103 | 94 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 39.70 | 114.04 | 271.73 | 35.93 | 116.38 | 286.16 |
| Standard deviation | 2.55 | 6.55 | 11.98 | 2.11 | 5.43 | 14.79 |
| CV (%) | 6.42 | 5.74 | 4.41 | 5.88 | 4.67 | 5.17 |