Mouse IL-12 p40 (Interleukin 12 p40) Sandwich ELISA Kit (STJE0019755)

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STJE0019755
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Short Description :This IL-12 p40 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
Applications:ELISA
Reactivity:Mouse
Sensitivity:4.69pg/mL
Detection Limit:7.81-500pg/mL
Note:FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Storage Instruction:If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:3.5h
Detection:Colormetric
Gene Symbol:Il12b
Gene ID:16160
Uniprot ID:IL12B_MOUSE
Specificity:This kit recognizes Mouse IL-12 p40 in samples. No significant cross-reactivity or interference between Mouse IL-12 p40 and analogues was observed.
Sample Type:Serum, plasma and other biological fluids
Protein Name Interleukin-12 Subunit Beta
Il-12b
Cytotoxic Lymphocyte Maturation Factor 40 Kda Subunit
Clmf P40
Il-12 Subunit P40
Database Links Reactome: R-MMU-9020591
Reactome: -MMU-9020933
Cellular Localisation Secreted
Alternative ELISA Names Interleukin-12 Subunit Beta ELISA kit
Il-12b ELISA kit
Cytotoxic Lymphocyte Maturation Factor 40 Kda Subunit ELISA kit
Clmf P40 ELISA kit
Il-12 Subunit P40 ELISA kit
Il12b ELISA kit
Specificity This kit recognizes Mouse IL-12 p40 in samples. No significant cross-reactivity or interference between Mouse IL-12 p40 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (50×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (50×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-104 95
EDTA plasma (n=8) 94-110 100
Cell culture media (n=8) 95-108 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 27.02 60.23 190.57 29.48 61.46 176.59
Standard deviation 1.80 3.23 7.58 1.96 2.85 5.40
CV (%) 6.65 5.37 3.98 6.66 4.63 3.06

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