Mouse IL-1 beta (IL-1 beta) Antibody Pair (STJAP00737)
General Information
| Short Description | This IL-1 beta antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R02-6W6/R05-7Z7 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | Il1b |
| Gene ID | 16176 |
| Uniprot ID | IL1B_MOUSE |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Expressed in activated macrophages (at protein level). |
| Function | Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. |
| Protein Name | Interleukin-1 BetaIl-1 Beta |
| Database Links | Reactome: R-MMU-448706Reactome: -MMU-5620971Reactome: -MMU-5660668Reactome: -MMU-9020702 |
| Cellular Localisation | CytoplasmCytosolSecretedLysosomeExtracellular ExosomeThe Precursor Is CytosolicIn Response To Inflammasome-Activating SignalsSuch As Atp For Nlrp3 Inflammasome Or Bacterial Flagellin For Nlrc4 InflammasomeCleaved And SecretedMature Form Is Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) PoreIn ContrastThe Precursor Form Is Not ReleasedDue To The Presence Of An Acidic Region That Is Proteolytically Removed By Casp1 During MaturationThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10 |
| Alternative Antibody Pair Names | Interleukin-1 Beta antibody pairIl-1 Beta antibody pairIl1b antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse IL-1 beta (IL-1 beta) Antibody Pair (STJAP00737)
This antibody pair is designed to detect mouse IL-1 beta (UniProt P10749) and has confirmed reactivity with mouse samples only. Both antibodies were raised against a recombinant protein immunogen.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated at 1 mg/mL in 0.01M PBS, pH 7.2, and purified by affinity chromatography. The clone IDs are R02-6W6 and R05-7Z7 respectively.
This antibody pair has been validated for use in ELISA. No other assay formats are specified in the product documentation.
Working dilutions for both antibodies are to be determined by the end user. Titration experiments are recommended to establish optimal concentrations for the specific experimental conditions and sample types being used.
Both components should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C. Repeated freeze/thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.