| Function | Multifunctional protein that plays a critical role in regulating the availability of IGFs to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Increases the cell proliferation of osteoblasts, intestinal smooth muscle cells and neuroblastoma cells. Enhances adhesion and survival of epithelial cells but decreases adhesion of mesenchymal cells. Once secreted, acts as a major mediator of mTORC1-dependent feedback inhibition of IGF1 signaling. Plays also a role in the induction of extracellular matrix (ECM) production and deposition independently of its nuclear translocation and binding to IGFs. Acts itself as a growth factor that can act independently of IGFs to regulate bone formation. Acts as a ligand for the ROR1 receptor which triggers formation of ROR1/HER2 heterodimer to enhance CREB oncogenic signaling. |
| Protein Name | Insulin-Like Growth Factor-Binding Protein 5Ibp-5Igf-Binding Protein 5Igfbp-5 |
| Database Links | Reactome: R-MMU-381426Reactome: -MMU-8957275 |
| Cellular Localisation | SecretedCytoplasmNucleus |
| Alternative ELISA Names | Insulin-Like Growth Factor-Binding Protein 5 ELISA kitIbp-5 ELISA kitIgf-Binding Protein 5 ELISA kitIgfbp-5 ELISA kitIgfbp5 ELISA kitIgfbp-5 ELISA kit |
| Specificity | This kit recognizes Mouse IGFBP-5 in samples. No significant cross-reactivity or interference between Mouse IGFBP-5 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-108 | 100 |
| EDTA plasma (n=8) | 85-98 | 91 |
| Cell culture media (n=8) | 93-107 | 100 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 403.20 | 775.71 | 3305.74 | 376.19 | 779.46 | 3571.63 |
| Standard deviation | 24.64 | 41.89 | 174.21 | 19.37 | 42.79 | 122.51 |
| CV (%) | 6.11 | 5.40 | 5.27 | 5.15 | 5.49 | 3.43 |
