Mouse IFN gamma (IFN gamma) Antibody Pair (STJAP00747)
General Information
| Short Description | This IFN gamma antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R06-5X9/R07-1C7 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | Ifng |
| Gene ID | 15978 |
| Uniprot ID | IFNG_MOUSE |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Released primarily from activated T lymphocytes. |
| Function | Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation. |
| Protein Name | Interferon GammaIfn-Gamma |
| Database Links | Reactome: R-MMU-877300Reactome: -MMU-877312Reactome: -MMU-9732724 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Interferon Gamma antibody pairIfn-Gamma antibody pairIfng antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse IFN gamma (IFN gamma) Antibody Pair (STJAP00747)
This antibody pair is designed to detect mouse IFN gamma (UniProt P01580) and has confirmed reactivity against mouse samples only. It is intended for use in sandwich ELISA formats with recombinant protein used as the immunogen.
This antibody pair has been validated for use in ELISA. No other assay formats are listed as confirmed applications for this product.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated at 1 mg/mL, formulated in 0.01M PBS at pH 7.2. They are affinity purified and carry clone IDs R06-5X9 and R07-1C7 respectively.
Working dilutions for both antibodies are to be determined by the end user and are not specified in the product documentation. Titration experiments are recommended to establish optimal concentrations for each specific experimental setup.
Both components should be stored at 4°C for short-term use. For long-term storage, aliquots should be kept at -20°C, and repeated freeze/thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.