| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by the ability of the immobilized protein to support the adhesion of THP-1 cells. When cells are added to ICAM-1 coated plates (12.5 Mu g/mL, 100 Mu L/well) , approximately >80% cells will adhere specifically after 60 minutes at 37 °C. |
| Gene Symbol: | Icam1 |
| Gene ID: | 15894 |
| Uniprot ID: | ICAM1_MOUSE |
| Immunogen: | Recombinant Mouse ICAM-1/CD54 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gln28-Asn485) of mouse ICAM-1/CD54 (Accession #NP_034623.1. ) fused with an Fc, 6×His tag at the C-terminus. |
| Immunogen Sequence: | QVSIHPREAFLPQGGSVQVN CSSSCKEDLSLGLETQWLKD ELESGPNWKLFELSEIGEDS SPLCFENCGTVQSSASATIT VYSFPESVELRPLPAWQQVG KDLTLRCHVDGGAPRTQLSA VLLRGEEILSRQPVGGHPKD PKEITFTVLASRGDHGANFS CRTELDLRPQGLALFSNVSE ARSLRTFDLPATIPKLDTPD LLEVGTQQKLFCSLEGLFPA SEARIYLELGGQMPTQEST |
| Post Translational Modifications | Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. |
| Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. |
| Protein Name | Intercellular Adhesion Molecule 1Icam-1Mala-2Myd10Cd Antigen Cd54 |
| Database Links | Reactome: R-MMU-198933Reactome: -MMU-216083 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Intercellular Adhesion Molecule 1 proteinIcam-1 proteinMala-2 proteinMyd10 proteinCd Antigen Cd54 proteinIcam1 proteinIcam-1 protein |
Information sourced from Uniprot.org