| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.94ng/mL |
| Detection Limit: | 1.56-100pg/mL |
| Short Description : | This HGF Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Hgf |
| Gene ID: | 15234 |
| Uniprot ID: | HGF_MOUSE |
| Specificity: | This kit recognizes Mouse HGF in samples. No significant cross-reactivity or interference between Mouse HGF and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. Activates MAPK signaling following TMPRSS13 cleavage and activation. |
| Protein Name | Hepatocyte Growth FactorHepatopoietin-AScatter FactorSf Cleaved Into - Hepatocyte Growth Factor Alpha Chain - Hepatocyte Growth Factor Beta Chain |
| Database Links | Reactome: R-MMU-114608Reactome: -MMU-1257604Reactome: -MMU-5673001Reactome: -MMU-6806942Reactome: -MMU-6807004Reactome: -MMU-6811558Reactome: -MMU-8851805Reactome: -MMU-8851907Reactome: -MMU-8865999Reactome: -MMU-8874081Reactome: -MMU-8875513Reactome: -MMU-8875555Reactome: -MMU-8875656Reactome: -MMU-8875791Reactome: -MMU-9734091 |
| Alternative ELISA Names | Hepatocyte Growth Factor ELISA kitHepatopoietin-A ELISA kitScatter Factor ELISA kitSf Cleaved Into - Hepatocyte Growth Factor Alpha Chain - Hepatocyte Growth Factor Beta Chain ELISA kitHgf ELISA kit |
| Specificity | This kit recognizes Mouse HGF in samples. No significant cross-reactivity or interference between Mouse HGF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 93-109 | 100 |
| EDTA plasma (n=8) | 93-106 | 100 |
| Cell culture media (n=8) | 94-107 | 99 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 4.70 | 9.77 | 35.91 | 4.98 | 9.39 | 38.13 |
| Standard deviation | 0.31 | 0.52 | 1.77 | 0.32 | 0.54 | 1.76 |
| CV (%) | 6.55 | 5.28 | 4.92 | 6.49 | 5.74 | 4.62 |