| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.064ng/mL |
| Detection Limit: | 0.156-10ng/mL |
| Short Description : | This HDAC6 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse tissue homogenates, cell lysates, cell culture supernates or other biological fluids.. |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | Hdac6 |
| Gene ID: | 15185 |
| Uniprot ID: | HDAC6_MOUSE |
| Sample Type: | tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Tissue Specificity | Expressed in neurons of the cortex. Expressed in Purkinje cells. Detected in keratinocytes (at protein level). |
| Post Translational Modifications | Phosphorylated by AURKA.phosphorylation increases HDAC6-mediated deacetylation of alpha-tubulin and subsequent disassembly of cilia. Ubiquitinated. Its polyubiquitination however does not lead to its degradation. Sumoylated in vitro. |
| Function | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. In addition to histones, deacetylates other proteins, such as CTTN, tubulin and SQSTM1. Plays a central role in microtubule-dependent cell motility by mediating deacetylation of tubulin. Required for cilia disassembly.via deacetylation of alpha-tubulin. Promotes deacetylation of CTTN, leading to actin polymerization, promotion of autophagosome-lysosome fusion and completion of autophagy. Promotes odontoblast differentiation following IPO7-mediated nuclear import and subsequent repression of RUNX2 expression. In addition to its protein deacetylase activity, plays a key role in the degradation of misfolded proteins: when misfolded proteins are too abundant to be degraded by the chaperone refolding system and the ubiquitin-proteasome, mediates the transport of misfolded proteins to a cytoplasmic juxtanuclear structure called aggresome. Probably acts as an adapter that recognizes polyubiquitinated misfolded proteins and target them to the aggresome, facilitating their clearance by autophagy. |
| Protein Name | Histone Deacetylase 6 Hd6 Histone Deacetylase Mhda2 Protein Deacetylase Hdac6 Tubulin-Lysine Deacetylase Hdac6 |
| Database Links | Reactome: R-MMU-3371511 Reactome: -MMU-350054 Reactome: -MMU-5617833 Reactome: -MMU-9646399 |
| Cellular Localisation | Cytoplasm Cytoskeleton Nucleus Perikaryon Cell Projection Dendrite Axon Cilium Microtubule Organizing Center Centrosome Cilium Basal Body It Is Mainly Cytoplasmic Where It Is Associated With Microtubules |
| Alternative ELISA Names | Histone Deacetylase 6 ELISA kit Hd6 ELISA kit Histone Deacetylase Mhda2 ELISA kit Protein Deacetylase Hdac6 ELISA kit Tubulin-Lysine Deacetylase Hdac6 ELISA kit Hdac6 ELISA kit |
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Information sourced from Uniprot.org