| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.049ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This H4 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Tissue Specificity | |
| Post Translational Modifications | Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin. Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation. Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by KMT5A/SET8. Trimethylation is performed by KMT5B and KMT5C and induces gene silencing. Monomethylated at Lys-13 (H4K12me1) by N6AMT1.H4K12me1 modification is present at the promoters of numerous genes encoding cell cycle regulators. Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4. Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me). Sumoylated, which is associated with transcriptional repression. Butyrylation of histones marks active promoters and competes with histone acetylation. It is present during late spermatogenesis. Hydroxybutyrylation of histones is induced by starvation. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. Glutarylation at Lys-92 (H4K91glu) destabilizes nucleosomes by promoting dissociation of the H2A-H2B dimers from nucleosomes. Ufmylated.monofmylated by UFL1 at Lys-32 (H4K31Ufm1) in response to DNA damage. Lactylated in macrophages by EP300/P300 by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription. |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Protein Name | Histone H4 |
| Database Links | Reactome: R-MMU-212300Reactome: -MMU-2299718Reactome: -MMU-3214841Reactome: -MMU-3214842Reactome: -MMU-3214847Reactome: -MMU-3214858Reactome: -MMU-4551638Reactome: -MMU-5693565Reactome: -MMU-5693571Reactome: -MMU-5693607Reactome: -MMU-606279Reactome: -MMU-69473Reactome: -MMU-8936459Reactome: -MMU-9018519 |
| Cellular Localisation | NucleusChromosome |
| Alternative ELISA Names | Histone H4 ELISA kitH4c1 ELISA kitHist1h4a.H4c2 ELISA kitH4-53 ELISA kitHist1h4b.H4c3 ELISA kitH4-12 ELISA kitHist1h4c.H4c4 ELISA kitHist1h4d.H4c6 ELISA kitHist1h4f.H4c8 ELISA kitHist1h4h.H4c9 ELISA kitHist1h4i.H4c11 ELISA kitHist1h4j.H4c12 ELISA kitHist1h4k.Hist1h4m.H4c14 ELISA kitHist2h4 ELISA kitHist2h4a.H4c16 ELISA kitH4f16 ELISA kitHist4h4 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
