Mouse GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) Sandwich ELISA Kit (STJE0019736)

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STJE0019736
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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.67pg/mL
Detection Limit: 7.81-500pg/mL
Short Description : This GM-CSF Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Csf2
Gene ID: 12981
Uniprot ID: CSF2_MOUSE
Specificity: This kit recognizes Mouse GM-CSF in samples. No significant cross-reactivity or interference between Mouse GM-CSF and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Granulocyte-Macrophage Colony-Stimulating Factor
Gm-Csf
Colony-Stimulating Factor
Csf
Database Links Reactome: R-MMU-512988
Reactome: -MMU-5673001
Reactome: -MMU-912526
Cellular Localisation Secreted
Alternative ELISA Names Granulocyte-Macrophage Colony-Stimulating Factor ELISA kit
Gm-Csf ELISA kit
Colony-Stimulating Factor ELISA kit
Csf ELISA kit
Csf2 ELISA kit
Csfgm ELISA kit
Specificity This kit recognizes Mouse GM-CSF in samples. No significant cross-reactivity or interference between Mouse GM-CSF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (50×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (50×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-102 93
EDTA plasma (n=8) 88-103 94
Cell culture media (n=8) 86-101 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 24.96 70.05 170.32 24.85 66.63 155.77
Standard deviation 1.30 3.34 7.78 1.69 3.57 6.85
CV (%) 5.19 4.77 4.57 6.79 5.36 4.40

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