Mouse GDH (Glutamate Dehydrogenase 1) Sandwich ELISA Kit (STJE0009126)
SPECIFICATIONS
General Information
| Short Description | This GDH Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma, tissue homogenates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Mouse |
| Sensitivity | 0.07U/L |
| Detection Limit | 0.156-10U/L |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 4.5 hrs |
Target Information
| Gene Symbol | Glud1 |
| Gene ID | 14661 |
| Uniprot ID | DHE3_MOUSE |
| Sample Type | serum, plasma, tissue homogenates or other biological fluids. |
Additional Info
| Tissue Specificity | |
| Post Translational Modifications | Acetylation of Lys-84 is observed in liver mitochondria from fasted mice but not from fed mice. ADP-ribosylated by SIRT4, leading to inactivate glutamate dehydrogenase activity. Stoichiometry shows that ADP-ribosylation occurs in one subunit per catalytically active homohexamer. |
| Function | Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. Plays a role in insulin homeostasis. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. |
| Protein Name | Glutamate Dehydrogenase 1 - MitochondrialGdh 1 |
| Database Links | Reactome: R-MMU-2151201Reactome: -MMU-8964539Reactome: -MMU-9837999 |
| Cellular Localisation | MitochondrionEndoplasmic ReticulumMostly Translocates Into The MitochondriaOnly A Small Amount Of The Protein Localizes To The Endoplasmic Reticulum |
| Alternative ELISA Names | Glutamate Dehydrogenase 1 - Mitochondrial ELISA kitGdh 1 ELISA kitGlud1 ELISA kitGlud ELISA kit |
| output |
Information sourced from Uniprot.org