| Function | Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. |
| Protein Name | Granulocyte Colony-Stimulating Factor
G-Csf |
| Database Links | Reactome: R-MMU-9674555
Reactome: -MMU-9705462 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Granulocyte Colony-Stimulating Factor ELISA kit
G-Csf ELISA kit
Csf3 ELISA kit
Csfg ELISA kit |
| Specificity | This kit recognizes Mouse G-CSF in samples. No significant cross-reactivity or interference between Mouse G-CSF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-106 | 97 |
| EDTA plasma (n=8) | 95-107 | 101 |
| Cell culture media (n=8) | 87-99 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 48.13 | 143.59 | 435.52 | 45.70 | 149.29 | 407.40 |
| Standard deviation | 2.51 | 7.48 | 16.55 | 2.38 | 6.97 | 21.80 |
| CV (%) | 5.21 | 5.21 | 3.80 | 5.20 | 4.67 | 5.35 |
